Detection of a new tet(X6)-encoding plasmid in Acinetobacter towneri
Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved..
OBJECTIVES: The aim of this study was to characterise a novel tet(X6)-carrying plasmid detected in a livestock-associated Acinetobacter towneri isolate.
METHODS: PCR screening was performed to detect tet(X) variants in livestock-associated Acinetobacter spp. isolates. The tet(X6)-positive isolate was analysed by whole-genome sequencing. Functional cloning was performed to detect the activity of Tet(X6). Antibiotic susceptibility was determined by broth dilution and microbiological degradation assays. Site-directed mutagenesis was performed to identify the role of 23-Ala residue of Tet(X6) in tigecycline resistance.
RESULTS: The tet(X6) gene was detected on a 159-kb plasmid (pAT205) carried by a tigecycline-susceptible A. towneri isolate recovered from a swine faecal sample. The genetic context of tet(X6) [ΔISVsa3-tet(X6)-abh-guaA-ISVsa3] is highly similar to that of the reported plasmid-borne tet(X) variants, suggesting that it may represent a common structure mediating the dissemination of plasmid-borne tet(X) genes. Additional resistance genes detected on pAT205 were carried by a Tn6205-like region and a disrupted class 2 integron. Gene expression and microbiological degradation assays consistently suggested that the activity of tet(X6) is weaker than that of tet(X3) and tet(X4). The 23-Ala residue of the first FAD-binding site conferred higher activity to Tet(X6) than the Gly reside conserved in the other plasmid-borne tet(X) variants, indicating that the site might be under selection.
CONCLUSION: This study alerts to the silent dissemination possibility of tigecycline resistance mediated by a novel plasmid. Continuous monitoring of plasmid-borne tet(X) is imperative for tackling its dissemination.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:25 |
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| Enthalten in: |
Journal of global antimicrobial resistance - 25(2021) vom: 28. Juni, Seite 132-136 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cheng, Yingying [VerfasserIn] |
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| Links: |
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| Themen: |
Acinetobacter towneri |
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| Anmerkungen: |
Date Completed 01.07.2021 Date Revised 01.07.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jgar.2021.03.004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM323182240 |
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| 041 | |a eng | ||
| 100 | 1 | |a Cheng, Yingying |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Detection of a new tet(X6)-encoding plasmid in Acinetobacter towneri |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Completed 01.07.2021 | ||
| 500 | |a Date Revised 01.07.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved. | ||
| 520 | |a OBJECTIVES: The aim of this study was to characterise a novel tet(X6)-carrying plasmid detected in a livestock-associated Acinetobacter towneri isolate | ||
| 520 | |a METHODS: PCR screening was performed to detect tet(X) variants in livestock-associated Acinetobacter spp. isolates. The tet(X6)-positive isolate was analysed by whole-genome sequencing. Functional cloning was performed to detect the activity of Tet(X6). Antibiotic susceptibility was determined by broth dilution and microbiological degradation assays. Site-directed mutagenesis was performed to identify the role of 23-Ala residue of Tet(X6) in tigecycline resistance | ||
| 520 | |a RESULTS: The tet(X6) gene was detected on a 159-kb plasmid (pAT205) carried by a tigecycline-susceptible A. towneri isolate recovered from a swine faecal sample. The genetic context of tet(X6) [ΔISVsa3-tet(X6)-abh-guaA-ISVsa3] is highly similar to that of the reported plasmid-borne tet(X) variants, suggesting that it may represent a common structure mediating the dissemination of plasmid-borne tet(X) genes. Additional resistance genes detected on pAT205 were carried by a Tn6205-like region and a disrupted class 2 integron. Gene expression and microbiological degradation assays consistently suggested that the activity of tet(X6) is weaker than that of tet(X3) and tet(X4). The 23-Ala residue of the first FAD-binding site conferred higher activity to Tet(X6) than the Gly reside conserved in the other plasmid-borne tet(X) variants, indicating that the site might be under selection | ||
| 520 | |a CONCLUSION: This study alerts to the silent dissemination possibility of tigecycline resistance mediated by a novel plasmid. Continuous monitoring of plasmid-borne tet(X) is imperative for tackling its dissemination | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Acinetobacter towneri | |
| 650 | 4 | |a Novel plasmid | |
| 650 | 4 | |a Plasmid-borne | |
| 650 | 4 | |a Tigecycline resistance | |
| 650 | 4 | |a tet(X6) | |
| 650 | 7 | |a Anti-Bacterial Agents |2 NLM | |
| 700 | 1 | |a Chen, Yong |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Jingjie |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yunbo |e verfasserin |4 aut | |
| 700 | 1 | |a Shan, Tongling |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Yonghong |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Kai |e verfasserin |4 aut | |
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