Unique diagnostic signatures of concussion in the saliva of male athletes : the Study of Concussion in Rugby Union through MicroRNAs (SCRUM)
© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ..
OBJECTIVE: To investigate the role of salivary small non-coding RNAs (sncRNAs) in the diagnosis of sport-related concussion.
METHODS: Saliva was obtained from male professional players in the top two tiers of England's elite rugby union competition across two seasons (2017-2019). Samples were collected preseason from 1028 players, and during standardised head injury assessments (HIAs) at three time points (in-game, post-game, and 36-48 hours post-game) from 156 of these. Samples were also collected from controls (102 uninjured players and 66 players sustaining a musculoskeletal injury). Diagnostic sncRNAs were identified with next generation sequencing and validated using quantitative PCR in 702 samples. A predictive logistic regression model was built on 2017-2018 data (training dataset) and prospectively validated the following season (test dataset).
RESULTS: The HIA process confirmed concussion in 106 players (HIA+) and excluded this in 50 (HIA-). 32 sncRNAs were significantly differentially expressed across these two groups, with let-7f-5p showing the highest area under the curve (AUC) at 36-48 hours. Additionally, a combined panel of 14 sncRNAs (let-7a-5p, miR-143-3p, miR-103a-3p, miR-34b-3p, RNU6-7, RNU6-45, Snora57, snoU13.120, tRNA18Arg-CCT, U6-168, U6-428, U6-1249, Uco22cjg1,YRNA_255) could differentiate concussed subjects from all other groups, including players who were HIA- and controls, immediately after the game (AUC 0.91, 95% CI 0.81 to 1) and 36-48 hours later (AUC 0.94, 95% CI 0.86 to 1). When prospectively tested, the panel confirmed high predictive accuracy (AUC 0.96, 95% CI 0.92 to 1 post-game and AUC 0.93, 95% CI 0.86 to 1 at 36-48 hours).
CONCLUSIONS: SCRUM, a large prospective observational study of non-invasive concussion biomarkers, has identified unique signatures of concussion in saliva of male athletes diagnosed with concussion.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
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| Enthalten in: |
British journal of sports medicine - 55(2021), 24 vom: 23. Dez., Seite 1395-1404 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Di Pietro, Valentina [VerfasserIn] |
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| Links: |
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| Themen: |
Brain |
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| Anmerkungen: |
Date Completed 03.12.2021 Date Revised 31.05.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1136/bjsports-2020-103274 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM323142230 |
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| 245 | 1 | 0 | |a Unique diagnostic signatures of concussion in the saliva of male athletes |b the Study of Concussion in Rugby Union through MicroRNAs (SCRUM) |
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| 500 | |a Date Completed 03.12.2021 | ||
| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY. Published by BMJ. | ||
| 520 | |a OBJECTIVE: To investigate the role of salivary small non-coding RNAs (sncRNAs) in the diagnosis of sport-related concussion | ||
| 520 | |a METHODS: Saliva was obtained from male professional players in the top two tiers of England's elite rugby union competition across two seasons (2017-2019). Samples were collected preseason from 1028 players, and during standardised head injury assessments (HIAs) at three time points (in-game, post-game, and 36-48 hours post-game) from 156 of these. Samples were also collected from controls (102 uninjured players and 66 players sustaining a musculoskeletal injury). Diagnostic sncRNAs were identified with next generation sequencing and validated using quantitative PCR in 702 samples. A predictive logistic regression model was built on 2017-2018 data (training dataset) and prospectively validated the following season (test dataset) | ||
| 520 | |a RESULTS: The HIA process confirmed concussion in 106 players (HIA+) and excluded this in 50 (HIA-). 32 sncRNAs were significantly differentially expressed across these two groups, with let-7f-5p showing the highest area under the curve (AUC) at 36-48 hours. Additionally, a combined panel of 14 sncRNAs (let-7a-5p, miR-143-3p, miR-103a-3p, miR-34b-3p, RNU6-7, RNU6-45, Snora57, snoU13.120, tRNA18Arg-CCT, U6-168, U6-428, U6-1249, Uco22cjg1,YRNA_255) could differentiate concussed subjects from all other groups, including players who were HIA- and controls, immediately after the game (AUC 0.91, 95% CI 0.81 to 1) and 36-48 hours later (AUC 0.94, 95% CI 0.86 to 1). When prospectively tested, the panel confirmed high predictive accuracy (AUC 0.96, 95% CI 0.92 to 1 post-game and AUC 0.93, 95% CI 0.86 to 1 at 36-48 hours) | ||
| 520 | |a CONCLUSIONS: SCRUM, a large prospective observational study of non-invasive concussion biomarkers, has identified unique signatures of concussion in saliva of male athletes diagnosed with concussion | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Observational Study | |
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| 650 | 4 | |a contact sports | |
| 650 | 4 | |a diagnosis | |
| 650 | 4 | |a trauma | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 700 | 1 | |a O'Halloran, Patrick |e verfasserin |4 aut | |
| 700 | 1 | |a Watson, Callum N |e verfasserin |4 aut | |
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| 700 | 1 | |a Yakoub, Kamal M |e verfasserin |4 aut | |
| 700 | 1 | |a Bentley, Conor |e verfasserin |4 aut | |
| 700 | 1 | |a Davies, David J |e verfasserin |4 aut | |
| 700 | 1 | |a Iliceto, Paolo |e verfasserin |4 aut | |
| 700 | 1 | |a Candilera, Gabriella |e verfasserin |4 aut | |
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| 700 | 1 | |a Stokes, Keith A |e verfasserin |4 aut | |
| 700 | 1 | |a Kemp, Simon Pt |e verfasserin |4 aut | |
| 700 | 1 | |a Belli, Antonio |e verfasserin |4 aut | |
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