Development of New Indonesian Propolis Extract-Loaded Self-emulsifying : Characterization, Stability and Antibacterial Activity
© 2021 The Authors..
Purpose: This study aimed to prepare, characterize, examine the stability and evaluation of the antibacterial activity of Indonesian propolis extract-loaded self-emulsifying (PESE). Methods: Oil, emulsifier, and co-emulsifier were selected as the carrier for the PESE formulation through a propolis-extract solubility test on each carrier, followed by evaluation of the nanoemulsion region in a pseudo ternary phase diagram. Pre-concentrate of PESE was prepared with the addition of 150 mg/mL propolis extract followed by characterization for the transmittance, globule size, zeta potential, thermodynamic stability, robustness to dilution, and accelerated stability. The selected formulation was tested for antibacterial activity using a microdilution method. Results: The PESE characterization produced a clear nanoemulsion with a globule size ranging from 13 to 45 nm and zeta potential of less than -38 mV. The PESE formulation with a composition of 150 mg/mL propolis extract, 20% castor oil, 40%-70% Kolliphor EL, and 10%-40% polyethylene glycol (PEG) 400 were thermodynamically stable. The PESE formulation with the composition of 20% castor oil, 40% Kolliphor EL, and 40% PEG 400 was the optimum formulation that passed the robustness to dilution evaluation and an accelerated stability test for 3 months. The antibacterial activity test on this formulation indicated improved activity against Escherichia coli and Staphylococcus aureus compared with that of propolis extract. Conclusion: These studies demonstrated that PESE in optimum formulation could be used as an antibacterial, particularly in E. coli and S. aureus.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Advanced pharmaceutical bulletin - 11(2021), 1 vom: 19. Jan., Seite 120-129 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Syukri, Yandi [VerfasserIn] |
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| Links: |
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| Themen: |
Antibacterial |
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| Anmerkungen: |
Date Revised 23.03.2021 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.34172/apb.2021.013 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM323042295 |
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| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2021 The Authors. | ||
| 520 | |a Purpose: This study aimed to prepare, characterize, examine the stability and evaluation of the antibacterial activity of Indonesian propolis extract-loaded self-emulsifying (PESE). Methods: Oil, emulsifier, and co-emulsifier were selected as the carrier for the PESE formulation through a propolis-extract solubility test on each carrier, followed by evaluation of the nanoemulsion region in a pseudo ternary phase diagram. Pre-concentrate of PESE was prepared with the addition of 150 mg/mL propolis extract followed by characterization for the transmittance, globule size, zeta potential, thermodynamic stability, robustness to dilution, and accelerated stability. The selected formulation was tested for antibacterial activity using a microdilution method. Results: The PESE characterization produced a clear nanoemulsion with a globule size ranging from 13 to 45 nm and zeta potential of less than -38 mV. The PESE formulation with a composition of 150 mg/mL propolis extract, 20% castor oil, 40%-70% Kolliphor EL, and 10%-40% polyethylene glycol (PEG) 400 were thermodynamically stable. The PESE formulation with the composition of 20% castor oil, 40% Kolliphor EL, and 40% PEG 400 was the optimum formulation that passed the robustness to dilution evaluation and an accelerated stability test for 3 months. The antibacterial activity test on this formulation indicated improved activity against Escherichia coli and Staphylococcus aureus compared with that of propolis extract. Conclusion: These studies demonstrated that PESE in optimum formulation could be used as an antibacterial, particularly in E. coli and S. aureus | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Antibacterial | |
| 650 | 4 | |a Castor oil | |
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