Application of high-resolution ultrasonic spectroscopy for detection of the plasmin activity toward β-casein
Copyright © 2021 Elsevier Ltd. All rights reserved..
High-resolution ultrasonic spectroscopy (HR-US) was applied for precise detection of plasmin activity towards β-casein in buffer at pH 7.8 and 37 °C. The evolution of ultrasonic velocity and ultrasonic attenuation measured at 15.5 MHz is related to the concentration of peptide bonds hydrolyzed and loss of β-casein aggregates, respectively. The ultrasonic assay presents sensitive and direct activity-based quantification of plasmin levels in milk. The variation in plasmin concentration between HR-US and ELISA method owed to the differing detection principles. The real-time ultrasonic profiles of hydrolysis were utilized to describe the kinetic aspect of plasmin activity. The non-linear activity curve was fitted with classic and inverse Michaelis-Menten type models. Within 1-8.6 mg·mL-1 β-casein, the Vmax and KM obtained were (6.30 ± 2.21) × 10-5 mol.kg-1·min-1 and 10.33 ± 3.50 mg·mL-1, respectively. The maximum peptide bond cleaved was 5-6 (2.7% degree of hydrolysis) achieved at 1 mg·mL-1 β-casein.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:353 |
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| Enthalten in: |
Food chemistry - 353(2021) vom: 15. Aug., Seite 129373 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Dizon, Mark [VerfasserIn] |
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| Links: |
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| Themen: |
β-Casein |
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| Anmerkungen: |
Date Completed 03.06.2021 Date Revised 03.06.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.foodchem.2021.129373 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM322872731 |
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| 520 | |a Copyright © 2021 Elsevier Ltd. All rights reserved. | ||
| 520 | |a High-resolution ultrasonic spectroscopy (HR-US) was applied for precise detection of plasmin activity towards β-casein in buffer at pH 7.8 and 37 °C. The evolution of ultrasonic velocity and ultrasonic attenuation measured at 15.5 MHz is related to the concentration of peptide bonds hydrolyzed and loss of β-casein aggregates, respectively. The ultrasonic assay presents sensitive and direct activity-based quantification of plasmin levels in milk. The variation in plasmin concentration between HR-US and ELISA method owed to the differing detection principles. The real-time ultrasonic profiles of hydrolysis were utilized to describe the kinetic aspect of plasmin activity. The non-linear activity curve was fitted with classic and inverse Michaelis-Menten type models. Within 1-8.6 mg·mL-1 β-casein, the Vmax and KM obtained were (6.30 ± 2.21) × 10-5 mol.kg-1·min-1 and 10.33 ± 3.50 mg·mL-1, respectively. The maximum peptide bond cleaved was 5-6 (2.7% degree of hydrolysis) achieved at 1 mg·mL-1 β-casein | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a High-resolution ultrasonic spectroscopy | |
| 650 | 4 | |a Hydrolysis | |
| 650 | 4 | |a Plasmin | |
| 650 | 4 | |a Ultrasonic velocity | |
| 650 | 4 | |a β-Casein | |
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