Reformulation of an extant ATPase active site to mimic ancestral GTPase activity reveals a nucleotide base requirement for function
Hydrolysis of nucleoside triphosphates releases similar amounts of energy. However, ATP hydrolysis is typically used for energy-intensive reactions, whereas GTP hydrolysis typically functions as a switch. SpoIVA is a bacterial cytoskeletal protein that hydrolyzes ATP to polymerize irreversibly during Bacillus subtilis sporulation. SpoIVA evolved from a TRAFAC class of P-loop GTPases, but the evolutionary pressure that drove this change in nucleotide specificity is unclear. We therefore reengineered the nucleotide-binding pocket of SpoIVA to mimic its ancestral GTPase activity. SpoIVAGTPase functioned properly as a GTPase but failed to polymerize because it did not form an NDP-bound intermediate that we report is required for polymerization. Further, incubation of SpoIVAGTPase with limiting ATP did not promote efficient polymerization. This approach revealed that the nucleotide base, in addition to the energy released from hydrolysis, can be critical in specific biological functions. We also present data suggesting that increased levels of ATP relative to GTP at the end of sporulation was the evolutionary pressure that drove the change in nucleotide preference in SpoIVA.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
eLife - 10(2021) vom: 11. März |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Updegrove, Taylor B [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 07.12.2021 Date Revised 23.07.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.7554/eLife.65845 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM322611393 |
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| 100 | 1 | |a Updegrove, Taylor B |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Reformulation of an extant ATPase active site to mimic ancestral GTPase activity reveals a nucleotide base requirement for function |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 07.12.2021 | ||
| 500 | |a Date Revised 23.07.2023 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Hydrolysis of nucleoside triphosphates releases similar amounts of energy. However, ATP hydrolysis is typically used for energy-intensive reactions, whereas GTP hydrolysis typically functions as a switch. SpoIVA is a bacterial cytoskeletal protein that hydrolyzes ATP to polymerize irreversibly during Bacillus subtilis sporulation. SpoIVA evolved from a TRAFAC class of P-loop GTPases, but the evolutionary pressure that drove this change in nucleotide specificity is unclear. We therefore reengineered the nucleotide-binding pocket of SpoIVA to mimic its ancestral GTPase activity. SpoIVAGTPase functioned properly as a GTPase but failed to polymerize because it did not form an NDP-bound intermediate that we report is required for polymerization. Further, incubation of SpoIVAGTPase with limiting ATP did not promote efficient polymerization. This approach revealed that the nucleotide base, in addition to the energy released from hydrolysis, can be critical in specific biological functions. We also present data suggesting that increased levels of ATP relative to GTP at the end of sporulation was the evolutionary pressure that drove the change in nucleotide preference in SpoIVA | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, N.I.H., Intramural | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a B. subtilis | |
| 650 | 4 | |a MreB | |
| 650 | 4 | |a SpoVM | |
| 650 | 4 | |a actin | |
| 650 | 4 | |a cell biology | |
| 650 | 4 | |a infectious disease | |
| 650 | 4 | |a microbiology | |
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| 650 | 4 | |a septins | |
| 650 | 4 | |a tubulin | |
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| 650 | 7 | |a spore-specific proteins, Bacillus |2 NLM | |
| 650 | 7 | |a Guanosine Triphosphate |2 NLM | |
| 650 | 7 | |a 86-01-1 |2 NLM | |
| 650 | 7 | |a Adenosine Triphosphate |2 NLM | |
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| 650 | 7 | |a Adenosine Triphosphatases |2 NLM | |
| 650 | 7 | |a EC 3.6.1.- |2 NLM | |
| 650 | 7 | |a GTP Phosphohydrolases |2 NLM | |
| 650 | 7 | |a EC 3.6.1.- |2 NLM | |
| 700 | 1 | |a Harke, Jailynn |e verfasserin |4 aut | |
| 700 | 1 | |a Anantharaman, Vivek |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Gopalan, Nikhil |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Di |e verfasserin |4 aut | |
| 700 | 1 | |a Piszczek, Grzegorz |e verfasserin |4 aut | |
| 700 | 1 | |a Stevenson, David M |e verfasserin |4 aut | |
| 700 | 1 | |a Amador-Noguez, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jue D |e verfasserin |4 aut | |
| 700 | 1 | |a Aravind, L |e verfasserin |4 aut | |
| 700 | 1 | |a Ramamurthi, Kumaran S |e verfasserin |4 aut | |
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