BASP1 labels neural stem cells in the neurogenic niches of mammalian brain
The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistochemical data showed that the NSC-6 antibody recognized NSCs in the developing and postnatal murine brains as well as in human brain organoids. Mass spectrometry revealed the identity of the NSC-6 epitope as brain abundant, membrane-attached signal protein 1 (BASP1), a signaling protein that plays a key role in neurite outgrowth and plasticity. Western blot analysis using the NSC-6 antibody demonstrated multiple BASP1 isoforms with varying degrees of expression and correlating with distinct developmental stages. Herein, we describe the expression of BASP1 in NSCs in the developing and postnatal mammalian brains and human brain organoids, and demonstrate that the NSC-6 antibody may be a useful marker of these cells.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Scientific reports - 11(2021), 1 vom: 10. März, Seite 5546 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Manganas, Louis N [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 09.12.2021 Date Revised 11.02.2024 published: Electronic ErratumIn: Sci Rep. 2021 Oct 4;11(1):20008. - PMID 34608270 Citation Status MEDLINE |
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| doi: |
10.1038/s41598-021-85129-1 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM322495113 |
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| 245 | 1 | 0 | |a BASP1 labels neural stem cells in the neurogenic niches of mammalian brain |
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| 500 | |a Date Revised 11.02.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a ErratumIn: Sci Rep. 2021 Oct 4;11(1):20008. - PMID 34608270 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The mechanisms responsible for determining neural stem cell fate are numerous and complex. To begin to identify the specific components involved in these processes, we generated several mouse neural stem cell (NSC) antibodies against cultured mouse embryonic neurospheres. Our immunohistochemical data showed that the NSC-6 antibody recognized NSCs in the developing and postnatal murine brains as well as in human brain organoids. Mass spectrometry revealed the identity of the NSC-6 epitope as brain abundant, membrane-attached signal protein 1 (BASP1), a signaling protein that plays a key role in neurite outgrowth and plasticity. Western blot analysis using the NSC-6 antibody demonstrated multiple BASP1 isoforms with varying degrees of expression and correlating with distinct developmental stages. Herein, we describe the expression of BASP1 in NSCs in the developing and postnatal mammalian brains and human brain organoids, and demonstrate that the NSC-6 antibody may be a useful marker of these cells | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Antigens, Differentiation |2 NLM | |
| 650 | 7 | |a Basp1 protein, mouse |2 NLM | |
| 650 | 7 | |a Calmodulin-Binding Proteins |2 NLM | |
| 650 | 7 | |a Cytoskeletal Proteins |2 NLM | |
| 650 | 7 | |a Nerve Tissue Proteins |2 NLM | |
| 700 | 1 | |a Durá, Irene |e verfasserin |4 aut | |
| 700 | 1 | |a Osenberg, Sivan |e verfasserin |4 aut | |
| 700 | 1 | |a Semerci, Fatih |e verfasserin |4 aut | |
| 700 | 1 | |a Tosun, Mehmet |e verfasserin |4 aut | |
| 700 | 1 | |a Mishra, Rachana |e verfasserin |4 aut | |
| 700 | 1 | |a Parkitny, Luke |e verfasserin |4 aut | |
| 700 | 1 | |a Encinas, Juan M |e verfasserin |4 aut | |
| 700 | 1 | |a Maletic-Savatic, Mirjana |e verfasserin |4 aut | |
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