Cell-extracellular matrix interactions in the fluidic phase direct the topology and polarity of self-organized epithelial structures
© 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd..
INTRODUCTION: In vivo, cells are surrounded by extracellular matrix (ECM). To build organs from single cells, it is generally believed that ECM serves as scaffolds to coordinate cell positioning and differentiation. Nevertheless, how cells utilize cell-ECM interactions for the spatiotemporal coordination to different ECM at the tissue scale is not fully understood.
METHODS: Here, using in vitro assay with engineered MDCK cells expressing H2B-mCherry (nucleus) and gp135/Podocalyxin-GFP (apical marker), we show in multi-dimensions that such coordination for epithelial morphogenesis can be determined by cell-soluble ECM interaction in the fluidic phase.
RESULTS: The coordination depends on the native topology of ECM components such as sheet-like basement membrane (BM) and type I collagen (COL) fibres: scaffold formed by BM (COL) facilitates a close-ended (open-ended) coordination that leads to the formation of lobular (tubular) epithelium. Further, cells form apicobasal polarity throughout the entire lobule/tubule without a complete coverage of ECM at the basal side, and time-lapse two-photon scanning imaging reveals the polarization occurring early and maintained through the lobular expansion. During polarization, gp135-GFP was converged to the apical surface collectively in the lobular/tubular structures, suggesting possible intercellular communications. Under suspension culture, the polarization was impaired with multi-lumen formation in the tubules, implying the importance of ECM biomechanical microenvironment.
CONCLUSION: Our results suggest a biophysical mechanism for cells to form polarity and coordinate positioning at tissue scale, and in engineering epithelium through cell-soluble ECM interaction and self-assembly.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:54 |
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| Enthalten in: |
Cell proliferation - 54(2021), 4 vom: 01. Apr., Seite e13014 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ouyang, Mingxing [VerfasserIn] |
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| Links: |
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| Themen: |
Cell-ECM interaction |
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| Anmerkungen: |
Date Completed 13.04.2021 Date Revised 13.04.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/cpr.13014 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM321741897 |
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| 500 | |a Date Revised 13.04.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd. | ||
| 520 | |a INTRODUCTION: In vivo, cells are surrounded by extracellular matrix (ECM). To build organs from single cells, it is generally believed that ECM serves as scaffolds to coordinate cell positioning and differentiation. Nevertheless, how cells utilize cell-ECM interactions for the spatiotemporal coordination to different ECM at the tissue scale is not fully understood | ||
| 520 | |a METHODS: Here, using in vitro assay with engineered MDCK cells expressing H2B-mCherry (nucleus) and gp135/Podocalyxin-GFP (apical marker), we show in multi-dimensions that such coordination for epithelial morphogenesis can be determined by cell-soluble ECM interaction in the fluidic phase | ||
| 520 | |a RESULTS: The coordination depends on the native topology of ECM components such as sheet-like basement membrane (BM) and type I collagen (COL) fibres: scaffold formed by BM (COL) facilitates a close-ended (open-ended) coordination that leads to the formation of lobular (tubular) epithelium. Further, cells form apicobasal polarity throughout the entire lobule/tubule without a complete coverage of ECM at the basal side, and time-lapse two-photon scanning imaging reveals the polarization occurring early and maintained through the lobular expansion. During polarization, gp135-GFP was converged to the apical surface collectively in the lobular/tubular structures, suggesting possible intercellular communications. Under suspension culture, the polarization was impaired with multi-lumen formation in the tubules, implying the importance of ECM biomechanical microenvironment | ||
| 520 | |a CONCLUSION: Our results suggest a biophysical mechanism for cells to form polarity and coordinate positioning at tissue scale, and in engineering epithelium through cell-soluble ECM interaction and self-assembly | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a cell-ECM interaction | |
| 650 | 4 | |a epithelial polarity | |
| 650 | 4 | |a epithelial self-assembly | |
| 650 | 4 | |a epithelial topology | |
| 650 | 4 | |a fluidic phase | |
| 650 | 4 | |a tubulogenesis | |
| 650 | 7 | |a Collagen Type I |2 NLM | |
| 650 | 7 | |a Gels |2 NLM | |
| 700 | 1 | |a Yu, Jiun-Yann |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Yenyu |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Linhong |e verfasserin |4 aut | |
| 700 | 1 | |a Guo, Chin-Lin |e verfasserin |4 aut | |
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