Cholangiocyte organoids can repair bile ducts after transplantation in the human liver
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works..
Organoid technology holds great promise for regenerative medicine but has not yet been applied to humans. We address this challenge using cholangiocyte organoids in the context of cholangiopathies, which represent a key reason for liver transplantation. Using single-cell RNA sequencing, we show that primary human cholangiocytes display transcriptional diversity that is lost in organoid culture. However, cholangiocyte organoids remain plastic and resume their in vivo signatures when transplanted back in the biliary tree. We then utilize a model of cell engraftment in human livers undergoing ex vivo normothermic perfusion to demonstrate that this property allows extrahepatic organoids to repair human intrahepatic ducts after transplantation. Our results provide proof of principle that cholangiocyte organoids can be used to repair human biliary epithelium.
| Errataetall: |
CommentIn: Science. 2021 Feb 19;371(6531):786-787. - PMID 33602846 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:371 |
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| Enthalten in: |
Science (New York, N.Y.) - 371(2021), 6531 vom: 19. Feb., Seite 839-846 |
| Sprache: |
Englisch |
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| Anmerkungen: |
Date Completed 08.03.2021 Date Revised 01.02.2023 published: Print CommentIn: Science. 2021 Feb 19;371(6531):786-787. - PMID 33602846 Citation Status MEDLINE |
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| doi: |
10.1126/science.aaz6964 |
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NLM321616499 |
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| 245 | 1 | 0 | |a Cholangiocyte organoids can repair bile ducts after transplantation in the human liver |
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| 500 | |a CommentIn: Science. 2021 Feb 19;371(6531):786-787. - PMID 33602846 | ||
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| 520 | |a Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. | ||
| 520 | |a Organoid technology holds great promise for regenerative medicine but has not yet been applied to humans. We address this challenge using cholangiocyte organoids in the context of cholangiopathies, which represent a key reason for liver transplantation. Using single-cell RNA sequencing, we show that primary human cholangiocytes display transcriptional diversity that is lost in organoid culture. However, cholangiocyte organoids remain plastic and resume their in vivo signatures when transplanted back in the biliary tree. We then utilize a model of cell engraftment in human livers undergoing ex vivo normothermic perfusion to demonstrate that this property allows extrahepatic organoids to repair human intrahepatic ducts after transplantation. Our results provide proof of principle that cholangiocyte organoids can be used to repair human biliary epithelium | ||
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