Saliva Pooling Strategy for the Large-Scale Detection of SARS-CoV-2, Through Working-Groups Testing of Asymptomatic Subjects for Potential Applications in Different Workplaces
Copyright © 2021 American College of Occupational and Environmental Medicine..
OBJECTIVE: To perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers.
METHODS: qRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2.
RESULTS: We found a high Cycle threshold correlation (r = 0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment.
CONCLUSION: Through this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:63 |
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| Enthalten in: |
Journal of occupational and environmental medicine - 63(2021), 7 vom: 01. Juli, Seite 541-547 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Girón-Pérez, Daniel Alberto [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Completed 20.07.2021 Date Revised 18.08.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1097/JOM.0000000000002176 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM321549694 |
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| 041 | |a eng | ||
| 100 | 1 | |a Girón-Pérez, Daniel Alberto |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Saliva Pooling Strategy for the Large-Scale Detection of SARS-CoV-2, Through Working-Groups Testing of Asymptomatic Subjects for Potential Applications in Different Workplaces |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 20.07.2021 | ||
| 500 | |a Date Revised 18.08.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 American College of Occupational and Environmental Medicine. | ||
| 520 | |a OBJECTIVE: To perform an improved large-scale SARS-CoV-2 detection on pooled tests of asymptomatic workers | ||
| 520 | |a METHODS: qRT-PCR validation of the SARS-CoV-2 detection in salivae samples and saliva pools and working-group saliva pooling and testing for SARS-CoV-2 | ||
| 520 | |a RESULTS: We found a high Cycle threshold correlation (r = 0.9099) between swabs and saliva samples. Then, through the pooling strategy, we detected that 18/360 (5%) of individual saliva samples were SARS-CoV-2 positive. Saliva-pooling efficiency (360 of test sample/30 individual PCR) was higher (5.45) than the reported for swabbing group-testing and we spared 82% of the PCR reagents as well as sampling and personal protection equipment | ||
| 520 | |a CONCLUSION: Through this simplified and less expensive procedure, we detected in a short time asymptomatic-infected SARS-CoV-2-carriers that were isolated from their co-workers, thus, this methodology can be implemented in different workplaces to ensure consumers that employees are not infectious | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 700 | 1 | |a Ruiz-Manzano, Rocío Alejandra |e verfasserin |4 aut | |
| 700 | 1 | |a Benitez-Trinidad, Alma Betsaida |e verfasserin |4 aut | |
| 700 | 1 | |a Ventura-Ramón, Guadalupe Herminia |e verfasserin |4 aut | |
| 700 | 1 | |a Covantes-Rosales, Carlos Eduardo |e verfasserin |4 aut | |
| 700 | 1 | |a Ojeda-Durán, Ansonny Jhovanny |e verfasserin |4 aut | |
| 700 | 1 | |a Mercado-Salgado, Ulises |e verfasserin |4 aut | |
| 700 | 1 | |a Toledo-Ibarra, Gladys Alejandra |e verfasserin |4 aut | |
| 700 | 1 | |a Díaz-Reséndiz, Karina Janice |e verfasserin |4 aut | |
| 700 | 1 | |a Girón-Pérez, Manuel Iván |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:63 |g year:2021 |g number:7 |g day:01 |g month:07 |g pages:541-547 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1097/JOM.0000000000002176 |3 Volltext |
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