Profiling plasma-extracellular vesicle proteins and microRNAs in diabetes onset in middle-aged male participants in the ELSA-Brasil study
© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society..
We measured plasma-derived extracellular vesicle (EV) proteins and their microRNA (miRNA) cargos in normoglycemic (NG), glucose intolerant (GI), and newly diagnosed diabetes mellitus (DM) in middle-aged male participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brazil). Mass spectrometry revealed decreased IGHG-1 and increased ITIH2 protein levels in the GI group compared with that in the NG group and higher serotransferrin in EVs in the DM group than in those in the NG and GI groups. The GI group also showed increased serum ferritin levels, as evaluated by biochemical analysis, compared with those in both groups. Seventeen miRNAs were differentially expressed (DEMiRs) in the plasma EVs of the three groups. DM patients showed upregulation of miR-141-3p and downregulation of miR-324-5p and -376c-3p compared with the NG and GI groups. The DM and GI groups showed increased miR-26b-5p expression compared with that in the NG group. The DM group showed decreased miR-374b-5p levels compared with those in the GI group and higher concentrations than those in the NG group. Thus, three EV proteins and five DEMiR cargos have potential prognostic importance for diabetic complications mainly associated with the immune function and iron status of GI and DM patients.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
Physiological reports - 9(2021), 3 vom: 05. Feb., Seite e14731 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Masi, Laureane N [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Blood Glucose |
|---|
| Anmerkungen: |
Date Completed 09.12.2021 Date Revised 10.11.2023 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.14814/phy2.14731 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM32146933X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM32146933X | ||
| 003 | DE-627 | ||
| 005 | 20231225175854.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.14814/phy2.14731 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1071.xml |
| 035 | |a (DE-627)NLM32146933X | ||
| 035 | |a (NLM)33587339 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Masi, Laureane N |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Profiling plasma-extracellular vesicle proteins and microRNAs in diabetes onset in middle-aged male participants in the ELSA-Brasil study |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 09.12.2021 | ||
| 500 | |a Date Revised 10.11.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society. | ||
| 520 | |a We measured plasma-derived extracellular vesicle (EV) proteins and their microRNA (miRNA) cargos in normoglycemic (NG), glucose intolerant (GI), and newly diagnosed diabetes mellitus (DM) in middle-aged male participants of the Brazilian Longitudinal Study of Adult Health (ELSA-Brazil). Mass spectrometry revealed decreased IGHG-1 and increased ITIH2 protein levels in the GI group compared with that in the NG group and higher serotransferrin in EVs in the DM group than in those in the NG and GI groups. The GI group also showed increased serum ferritin levels, as evaluated by biochemical analysis, compared with those in both groups. Seventeen miRNAs were differentially expressed (DEMiRs) in the plasma EVs of the three groups. DM patients showed upregulation of miR-141-3p and downregulation of miR-324-5p and -376c-3p compared with the NG and GI groups. The DM and GI groups showed increased miR-26b-5p expression compared with that in the NG group. The DM group showed decreased miR-374b-5p levels compared with those in the GI group and higher concentrations than those in the NG group. Thus, three EV proteins and five DEMiR cargos have potential prognostic importance for diabetic complications mainly associated with the immune function and iron status of GI and DM patients | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Multicenter Study | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a epigenetics | |
| 650 | 4 | |a glucose intolerance | |
| 650 | 4 | |a hyperglycemia | |
| 650 | 4 | |a proteomic | |
| 650 | 7 | |a Blood Glucose |2 NLM | |
| 650 | 7 | |a Blood Proteins |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 650 | 7 | |a Proteome |2 NLM | |
| 700 | 1 | |a Lotufo, Paulo A |e verfasserin |4 aut | |
| 700 | 1 | |a Ferreira, Frederico M |e verfasserin |4 aut | |
| 700 | 1 | |a Rodrigues, Alice C |e verfasserin |4 aut | |
| 700 | 1 | |a Serdan, Tamires D A |e verfasserin |4 aut | |
| 700 | 1 | |a Souza-Siqueira, Talita |e verfasserin |4 aut | |
| 700 | 1 | |a Braga, Aécio A |e verfasserin |4 aut | |
| 700 | 1 | |a Saldarriaga, Magda E G |e verfasserin |4 aut | |
| 700 | 1 | |a Alba-Loureiro, Tatiana C |e verfasserin |4 aut | |
| 700 | 1 | |a Borges, Fernanda T |e verfasserin |4 aut | |
| 700 | 1 | |a Cury, Diego P |e verfasserin |4 aut | |
| 700 | 1 | |a Hirata, Mario H |e verfasserin |4 aut | |
| 700 | 1 | |a Gorjão, Renata |e verfasserin |4 aut | |
| 700 | 1 | |a Pithon-Curi, Tania C |e verfasserin |4 aut | |
| 700 | 1 | |a Lottenberg, Simão A |e verfasserin |4 aut | |
| 700 | 1 | |a Fedeli, Ligia M G |e verfasserin |4 aut | |
| 700 | 1 | |a Nakaya, Helder T I |e verfasserin |4 aut | |
| 700 | 1 | |a Bensenor, Isabela J M |e verfasserin |4 aut | |
| 700 | 1 | |a Curi, Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Hirabara, Sandro M |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Physiological reports |d 2013 |g 9(2021), 3 vom: 05. Feb., Seite e14731 |w (DE-627)NLM228197236 |x 2051-817X |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2021 |g number:3 |g day:05 |g month:02 |g pages:e14731 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.14814/phy2.14731 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2021 |e 3 |b 05 |c 02 |h e14731 | ||