A quantitative in vivo assay for craniofacial developmental toxicity of histone deacetylases
Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved..
Many bony features of the face develop from endochondral ossification of preexisting collagen-rich cartilage structures. The proper development of these cartilage structures is essential to the morphological formation of the face. The developmental programs governing the formation of the pre-bone facial cartilages are sensitive to chemical compounds that disturb histone acetylation patterns and chromatin structure. We have taken advantage of this fact to develop a quantitative morphological assay of craniofacial developmental toxicity based on the distortion and deterioration of facial cartilage structures in zebrafish larvae upon exposure to increasing concentrations of several well-described histone deacetylase inhibitors. In this assay, we measure the angle formed by the developing ceratohyal bone as a precise, sensitive and quantitative proxy for the overall developmental status of facial cartilages. Using the well-established developmental toxicant and histone deacetylase-inhibiting compound valproic acid along with 12 structurally related compounds, we demonstrate the applicability of the ceratohyal angle assay to investigate structure-activity relationships.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:342 |
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| Enthalten in: |
Toxicology letters - 342(2021) vom: 15. Mai, Seite 20-25 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Koch, Bjørn E V [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 22.03.2021 Date Revised 13.12.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.toxlet.2021.02.005 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 100 | 1 | |a Koch, Bjørn E V |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A quantitative in vivo assay for craniofacial developmental toxicity of histone deacetylases |
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| 500 | |a Date Revised 13.12.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a Many bony features of the face develop from endochondral ossification of preexisting collagen-rich cartilage structures. The proper development of these cartilage structures is essential to the morphological formation of the face. The developmental programs governing the formation of the pre-bone facial cartilages are sensitive to chemical compounds that disturb histone acetylation patterns and chromatin structure. We have taken advantage of this fact to develop a quantitative morphological assay of craniofacial developmental toxicity based on the distortion and deterioration of facial cartilage structures in zebrafish larvae upon exposure to increasing concentrations of several well-described histone deacetylase inhibitors. In this assay, we measure the angle formed by the developing ceratohyal bone as a precise, sensitive and quantitative proxy for the overall developmental status of facial cartilages. Using the well-established developmental toxicant and histone deacetylase-inhibiting compound valproic acid along with 12 structurally related compounds, we demonstrate the applicability of the ceratohyal angle assay to investigate structure-activity relationships | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Craniofacial development | |
| 650 | 4 | |a Developmental and reproductive toxicology | |
| 650 | 4 | |a Embryonic development | |
| 650 | 4 | |a Histone deacetylase inhibition | |
| 650 | 4 | |a Neural crest | |
| 650 | 4 | |a Osteogenesis | |
| 650 | 4 | |a Toxicity assay | |
| 650 | 4 | |a Valproic acid | |
| 650 | 4 | |a Zebrafish | |
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| 650 | 7 | |a Anticonvulsants |2 NLM | |
| 650 | 7 | |a Antifungal Agents |2 NLM | |
| 650 | 7 | |a Butyrates |2 NLM | |
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| 700 | 1 | |a Meijer, Annemarie H |e verfasserin |4 aut | |
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