Bleach enhancement of mixed wood pulp by mixture of thermo-alkali-stable xylanase and mannanase derived through co-culturing of Alkalophilic Bacillus sp. NG-27 and Bacillus nealsonii PN-11
© 2020 Published by Elsevier Ltd..
The Application of a combination of enzymes is the best alternative to reduce the use of chemicals in the paper industry. Bacillus sp. NG-27 and Bacillus nealsonii PN-11 are known to produce thermoalkali stable xylanse (X) and mannanase (M) respectively having potential for pulp biobleaching. The Present study, reports the production of a mixture of X + M by co-culturing of strains in SSF and standardizing its application for pulp biobleaching. Production of enzymes by co-cultivation in SSF was optimized by statistical methods. Substantial increase in the yield of enzymes; 3.61 fold of xylanase and 37.71 fold of mannanase was achieved. Application of enzyme cocktail for pulp biobleaching resulted in a 45.64% reduction of kappa number with 55 IU g-1odp of enzyme dose (xylanase:mannanase; 3:1) at pH 8.0 in 1h at 65 °C along with significant increase in brightness (11%) and whiteness (75%). The Same quality of paper as made up from chemical treated pulp can be made from enzyme-treated pulp with 30% less use of chlorine. Structural analysis of enzyme-treated pulp showed dissolution of hemicellulose as indicated by pores, cracks and increased roughness all over the surface. Cocktail of X + M produced economically in a single fermentation having all the requisite characteristics for pulp biobleaching is a highly suitable candidate for application in the pulp and paper industry.
Errataetall: |
ErratumIn: Heliyon. 2021 Jun 04;7(6):e07142. - PMID 34141918 |
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Medienart: |
E-Artikel |
Erscheinungsjahr: |
2021 |
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Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
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Enthalten in: |
Heliyon - 7(2021), 1 vom: 27. Jan., Seite e05673 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Angural, Steffy [VerfasserIn] |
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Links: |
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Themen: |
Biobleaching |
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Anmerkungen: |
Date Revised 18.06.2021 published: Electronic-eCollection ErratumIn: Heliyon. 2021 Jun 04;7(6):e07142. - PMID 34141918 Citation Status PubMed-not-MEDLINE |
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doi: |
10.1016/j.heliyon.2020.e05673 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM321142306 |
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520 | |a © 2020 Published by Elsevier Ltd. | ||
520 | |a The Application of a combination of enzymes is the best alternative to reduce the use of chemicals in the paper industry. Bacillus sp. NG-27 and Bacillus nealsonii PN-11 are known to produce thermoalkali stable xylanse (X) and mannanase (M) respectively having potential for pulp biobleaching. The Present study, reports the production of a mixture of X + M by co-culturing of strains in SSF and standardizing its application for pulp biobleaching. Production of enzymes by co-cultivation in SSF was optimized by statistical methods. Substantial increase in the yield of enzymes; 3.61 fold of xylanase and 37.71 fold of mannanase was achieved. Application of enzyme cocktail for pulp biobleaching resulted in a 45.64% reduction of kappa number with 55 IU g-1odp of enzyme dose (xylanase:mannanase; 3:1) at pH 8.0 in 1h at 65 °C along with significant increase in brightness (11%) and whiteness (75%). The Same quality of paper as made up from chemical treated pulp can be made from enzyme-treated pulp with 30% less use of chlorine. Structural analysis of enzyme-treated pulp showed dissolution of hemicellulose as indicated by pores, cracks and increased roughness all over the surface. Cocktail of X + M produced economically in a single fermentation having all the requisite characteristics for pulp biobleaching is a highly suitable candidate for application in the pulp and paper industry | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Biobleaching | |
650 | 4 | |a Biological sciences | |
650 | 4 | |a Biotechnology | |
650 | 4 | |a Co-culturing | |
650 | 4 | |a Environmental science | |
650 | 4 | |a Enzymology | |
650 | 4 | |a Mannanase | |
650 | 4 | |a Microbiology | |
650 | 4 | |a Optimization | |
650 | 4 | |a Solid-state fermentation | |
650 | 4 | |a Xylanase | |
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700 | 1 | |a Kumar, Aditya |e verfasserin |4 aut | |
700 | 1 | |a Kumar, Deepak |e verfasserin |4 aut | |
700 | 1 | |a Jassal, Sunena |e verfasserin |4 aut | |
700 | 1 | |a Gupta, Naveen |e verfasserin |4 aut | |
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