Mucosal-Associated Invariant T (MAIT) Cells Are Highly Activated and Functionally Impaired in COVID-19 Patients
Coronavirus disease 2019 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), comprises mild courses of disease as well as progression to severe disease, characterised by lung and other organ failure. The immune system is considered to play a crucial role for the pathogenesis of COVID-19, although especially the contribution of innate-like T cells remains poorly understood. Here, we analysed the phenotype and function of mucosal-associated invariant T (MAIT) cells, innate-like T cells with potent antimicrobial effector function, in patients with mild and severe COVID-19 by multicolour flow cytometry. Our data indicate that MAIT cells are highly activated in patients with COVID-19, irrespective of the course of disease, and express high levels of proinflammatory cytokines such as IL-17A and TNFα ex vivo. Of note, expression of the activation marker HLA-DR positively correlated with SAPS II score, a measure of disease severity. Upon MAIT cell-specific in vitro stimulation, MAIT cells however failed to upregulate expression of the cytokines IL-17A and TNFα, as well as cytolytic proteins, that is, granzyme B and perforin. Thus, our data point towards an altered cytokine expression profile alongside an impaired antibacterial and antiviral function of MAIT cells in COVID-19 and thereby contribute to the understanding of COVID-19 immunopathogenesis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Viruses - 13(2021), 2 vom: 03. Feb. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Deschler, Sebastian [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 17.02.2021 Date Revised 10.11.2023 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/v13020241 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM321070623 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Coronavirus disease 2019 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), comprises mild courses of disease as well as progression to severe disease, characterised by lung and other organ failure. The immune system is considered to play a crucial role for the pathogenesis of COVID-19, although especially the contribution of innate-like T cells remains poorly understood. Here, we analysed the phenotype and function of mucosal-associated invariant T (MAIT) cells, innate-like T cells with potent antimicrobial effector function, in patients with mild and severe COVID-19 by multicolour flow cytometry. Our data indicate that MAIT cells are highly activated in patients with COVID-19, irrespective of the course of disease, and express high levels of proinflammatory cytokines such as IL-17A and TNFα ex vivo. Of note, expression of the activation marker HLA-DR positively correlated with SAPS II score, a measure of disease severity. Upon MAIT cell-specific in vitro stimulation, MAIT cells however failed to upregulate expression of the cytokines IL-17A and TNFα, as well as cytolytic proteins, that is, granzyme B and perforin. Thus, our data point towards an altered cytokine expression profile alongside an impaired antibacterial and antiviral function of MAIT cells in COVID-19 and thereby contribute to the understanding of COVID-19 immunopathogenesis | ||
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Erber, Johanna |e verfasserin |4 aut | |
| 700 | 1 | |a Fricke, Lisa |e verfasserin |4 aut | |
| 700 | 1 | |a Koyumdzhieva, Plamena |e verfasserin |4 aut | |
| 700 | 1 | |a Georgieva, Alexandra |e verfasserin |4 aut | |
| 700 | 1 | |a Lahmer, Tobias |e verfasserin |4 aut | |
| 700 | 1 | |a Wiessner, Johannes R |e verfasserin |4 aut | |
| 700 | 1 | |a Voit, Florian |e verfasserin |4 aut | |
| 700 | 1 | |a Schneider, Jochen |e verfasserin |4 aut | |
| 700 | 1 | |a Horstmann, Julia |e verfasserin |4 aut | |
| 700 | 1 | |a Iakoubov, Roman |e verfasserin |4 aut | |
| 700 | 1 | |a Treiber, Matthias |e verfasserin |4 aut | |
| 700 | 1 | |a Winter, Christof |e verfasserin |4 aut | |
| 700 | 1 | |a Ruland, Jürgen |e verfasserin |4 aut | |
| 700 | 1 | |a Busch, Dirk H |e verfasserin |4 aut | |
| 700 | 1 | |a Knolle, Percy A |e verfasserin |4 aut | |
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| 700 | 1 | |a Spinner, Christoph D |e verfasserin |4 aut | |
| 700 | 1 | |a Schmid, Roland M |e verfasserin |4 aut | |
| 700 | 1 | |a Quante, Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Böttcher, Katrin |e verfasserin |4 aut | |
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