TCR+/BCR+ dual-expressing cells and their associated public BCR clonotype are not enriched in type 1 diabetes
Copyright © 2020 Elsevier Inc. All rights reserved..
Ahmed and colleagues recently described a novel hybrid lymphocyte expressing both a B and T cell receptor, termed double expresser (DE) cells. DE cells in blood of type 1 diabetes (T1D) subjects were present at increased numbers and enriched for a public B cell clonotype. Here, we attempted to reproduce these findings. While we could identify DE cells by flow cytometry, we found no association between DE cell frequency and T1D status. We were unable to identify the reported public B cell clone, or any similar clone, in bulk B cells or sorted DE cells from T1D subjects or controls. We also did not observe increased usage of the public clone VH or DH genes in B cells or in sorted DE cells. Taken together, our findings suggest that DE cells and their alleged public clonotype are not enriched in T1D. This Matters Arising paper is in response to Ahmed et al. (2019), published in Cell. See also the response by Ahmed et al. (2021), published in this issue.
| Errataetall: |
CommentOn: Cell. 2019 May 30;177(6):1583-1599.e16. - PMID 31150624 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:184 |
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| Enthalten in: |
Cell - 184(2021), 3 vom: 04. Feb., Seite 827-839.e14 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Japp, Alberto Sada [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.08.2021 Date Revised 05.02.2022 published: Print CommentOn: Cell. 2019 May 30;177(6):1583-1599.e16. - PMID 31150624 Citation Status MEDLINE |
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| doi: |
10.1016/j.cell.2020.11.035 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM321056094 |
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| 500 | |a CommentOn: Cell. 2019 May 30;177(6):1583-1599.e16. - PMID 31150624 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Inc. All rights reserved. | ||
| 520 | |a Ahmed and colleagues recently described a novel hybrid lymphocyte expressing both a B and T cell receptor, termed double expresser (DE) cells. DE cells in blood of type 1 diabetes (T1D) subjects were present at increased numbers and enriched for a public B cell clonotype. Here, we attempted to reproduce these findings. While we could identify DE cells by flow cytometry, we found no association between DE cell frequency and T1D status. We were unable to identify the reported public B cell clone, or any similar clone, in bulk B cells or sorted DE cells from T1D subjects or controls. We also did not observe increased usage of the public clone VH or DH genes in B cells or in sorted DE cells. Taken together, our findings suggest that DE cells and their alleged public clonotype are not enriched in T1D. This Matters Arising paper is in response to Ahmed et al. (2019), published in Cell. See also the response by Ahmed et al. (2021), published in this issue | ||
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