Time course of the sensitivity and specificity of anti-SARS-CoV-2 IgM and IgG antibodies for symptomatic COVID-19 in Japan
The accurate and prompt diagnosis of SARS-CoV-2 infection is required for the control and treatment of the coronavirus infection disease 2019 (COVID-19). In this study, we aimed to investigate the time courses of the anti-severe acute corona respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM and IgG titers and to evaluate the sensitivity and specificity of such tests according to the specific day after the onset of COVID-19 among a patient population in Japan. We measured the titers of SARS-CoV-2 IgM and IgG in sera from 105 subjects, including 26 symptomatic COVID-19 patients, using chemiluminescent immunoassay (CLIA) methods utilizing magnetic beads coated with SARS-CoV-2 nucleocapsid protein and spike protein. The results of a ROC analysis suggested the possibility that the cutoff values in Japan might be lower than the manufacturer's reported cutoff (10 AU/mL): 1 AU/mL for IgM and 5 AU/mL for IgG. The sensitivity of the test before Day 8 after symptom onset was less than 50%; at Days 9-10, however, we obtained a much higher sensitivity of 81.8% for both IgM and IgG. At 15 days or later after symptom onset, the SARS-CoV-2 IgG test had a sensitivity of 100%. These results suggest that if the number of days since disease onset is taken into consideration, these antibody tests could be very useful for the diagnosis of COVID-19 and similar diseases.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Scientific reports - 11(2021), 1 vom: 02. Feb., Seite 2776 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Nakano, Yuki [VerfasserIn] |
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| Links: |
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| Themen: |
Immunoglobulin G |
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| Anmerkungen: |
Date Completed 19.02.2021 Date Revised 19.02.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41598-021-82428-5 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM320924556 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The accurate and prompt diagnosis of SARS-CoV-2 infection is required for the control and treatment of the coronavirus infection disease 2019 (COVID-19). In this study, we aimed to investigate the time courses of the anti-severe acute corona respiratory syndrome coronavirus 2 (SARS-CoV-2) IgM and IgG titers and to evaluate the sensitivity and specificity of such tests according to the specific day after the onset of COVID-19 among a patient population in Japan. We measured the titers of SARS-CoV-2 IgM and IgG in sera from 105 subjects, including 26 symptomatic COVID-19 patients, using chemiluminescent immunoassay (CLIA) methods utilizing magnetic beads coated with SARS-CoV-2 nucleocapsid protein and spike protein. The results of a ROC analysis suggested the possibility that the cutoff values in Japan might be lower than the manufacturer's reported cutoff (10 AU/mL): 1 AU/mL for IgM and 5 AU/mL for IgG. The sensitivity of the test before Day 8 after symptom onset was less than 50%; at Days 9-10, however, we obtained a much higher sensitivity of 81.8% for both IgM and IgG. At 15 days or later after symptom onset, the SARS-CoV-2 IgG test had a sensitivity of 100%. These results suggest that if the number of days since disease onset is taken into consideration, these antibody tests could be very useful for the diagnosis of COVID-19 and similar diseases | ||
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| 700 | 1 | |a Morita, Yoshifumi |e verfasserin |4 aut | |
| 700 | 1 | |a Shimura, Takuya |e verfasserin |4 aut | |
| 700 | 1 | |a Yokoyama, Rin |e verfasserin |4 aut | |
| 700 | 1 | |a Qian, Chungen |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Fuzhen |e verfasserin |4 aut | |
| 700 | 1 | |a He, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Kishi, Yoshiro |e verfasserin |4 aut | |
| 700 | 1 | |a Okada, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshikawa, Naoyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Nagura, Yutaka |e verfasserin |4 aut | |
| 700 | 1 | |a Okazaki, Hitoshi |e verfasserin |4 aut | |
| 700 | 1 | |a Moriya, Kyoji |e verfasserin |4 aut | |
| 700 | 1 | |a Seto, Yasuyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Kodama, Tatsuhiko |e verfasserin |4 aut | |
| 700 | 1 | |a Yatomi, Yutaka |e verfasserin |4 aut | |
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