Calcineurin subunit B is involved in shell regeneration in Haliotis diversicolor
©2021 Buddawong et al..
Abalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
|---|---|
| Enthalten in: |
PeerJ - 9(2021) vom: 01., Seite e10662 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Buddawong, Tiranan [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Biomineralization |
|---|
| Anmerkungen: |
Date Revised 02.02.2021 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.7717/peerj.10662 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM320816559 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM320816559 | ||
| 003 | DE-627 | ||
| 005 | 20231225174428.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.7717/peerj.10662 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1069.xml |
| 035 | |a (DE-627)NLM320816559 | ||
| 035 | |a (NLM)33520456 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Buddawong, Tiranan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Calcineurin subunit B is involved in shell regeneration in Haliotis diversicolor |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 02.02.2021 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a ©2021 Buddawong et al. | ||
| 520 | |a Abalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Biomineralization | |
| 650 | 4 | |a Calcineurin | |
| 650 | 4 | |a Haliotis diversicolor | |
| 650 | 4 | |a Regeneration | |
| 650 | 4 | |a Shell | |
| 700 | 1 | |a Asuvapongpatana, Somluk |e verfasserin |4 aut | |
| 700 | 1 | |a Suwannasing, Chanyatip |e verfasserin |4 aut | |
| 700 | 1 | |a Habuddha, Valainipha |e verfasserin |4 aut | |
| 700 | 1 | |a Sukonset, Chompoonut |e verfasserin |4 aut | |
| 700 | 1 | |a Sombutkayasith, Chanyarak |e verfasserin |4 aut | |
| 700 | 1 | |a McDougall, Carmel |e verfasserin |4 aut | |
| 700 | 1 | |a Weerachatyanukul, Wattana |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t PeerJ |d 2013 |g 9(2021) vom: 01., Seite e10662 |w (DE-627)NLM227171578 |x 2167-8359 |7 nnns |
| 773 | 1 | 8 | |g volume:9 |g year:2021 |g day:01 |g pages:e10662 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.7717/peerj.10662 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 9 |j 2021 |b 01 |h e10662 | ||