An ultrasensitive, homogeneous fluorescence quenching immunoassay integrating separation and detection of aflatoxin M1 based on magnetic graphene composites
A homogeneous fluorescence quenching immunoassay is described for simultaneous separation and detection of aflatoxin M1 (AFM1) in milk. The novel assay relies on monoclonal antibody (mAb) functionalized Fe3O4 decorated reduced-graphene oxide (rGO-Fe3O4-mAb) as both capture probe and energy acceptor, combined with tetramethylrhodamine cadaverine-labeled aflatoxin B1 (AFB1-TRCA) as the energy donor. In the assay, AFB1-TRCA binds to rGO-Fe3O4-mAb in the absence of AFM1, quenching the fluorescence of TRCA by resonance energy transfer. Significantly, the immunoassay integrates sample preparation and detection into a single step, by using magnetic graphene composites to avoid washing and centrifugation steps, and the assay can be completed within 10 min. Under optimized conditions, the visual and quantitative detection limits of the assay for AFM1 were 50 and 3.8 ng L-1, respectively, which were significantly lower than those obtained by fluorescence polarization immunoassay using the same immunoreagents. Owing to its operation and highly sensitivity, the proposed assay provides a powerful tool for the detection of AFM1.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:188 |
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| Enthalten in: |
Mikrochimica acta - 188(2021), 2 vom: 28. Jan., Seite 59 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Xiya [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.12.2021 Date Revised 15.12.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00604-021-04715-2 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM320690571 |
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| 100 | 1 | |a Zhang, Xiya |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An ultrasensitive, homogeneous fluorescence quenching immunoassay integrating separation and detection of aflatoxin M1 based on magnetic graphene composites |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a Date Completed 15.12.2021 | ||
| 500 | |a Date Revised 15.12.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A homogeneous fluorescence quenching immunoassay is described for simultaneous separation and detection of aflatoxin M1 (AFM1) in milk. The novel assay relies on monoclonal antibody (mAb) functionalized Fe3O4 decorated reduced-graphene oxide (rGO-Fe3O4-mAb) as both capture probe and energy acceptor, combined with tetramethylrhodamine cadaverine-labeled aflatoxin B1 (AFB1-TRCA) as the energy donor. In the assay, AFB1-TRCA binds to rGO-Fe3O4-mAb in the absence of AFM1, quenching the fluorescence of TRCA by resonance energy transfer. Significantly, the immunoassay integrates sample preparation and detection into a single step, by using magnetic graphene composites to avoid washing and centrifugation steps, and the assay can be completed within 10 min. Under optimized conditions, the visual and quantitative detection limits of the assay for AFM1 were 50 and 3.8 ng L-1, respectively, which were significantly lower than those obtained by fluorescence polarization immunoassay using the same immunoreagents. Owing to its operation and highly sensitivity, the proposed assay provides a powerful tool for the detection of AFM1 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Aflatoxin M1 | |
| 650 | 4 | |a Fluorescence quenching immunoassay | |
| 650 | 4 | |a Fluorescence resonance energy transfer | |
| 650 | 4 | |a Integrated sample treatment | |
| 650 | 4 | |a Magnetic graphene composites | |
| 650 | 7 | |a Antibodies, Immobilized |2 NLM | |
| 650 | 7 | |a Antibodies, Monoclonal |2 NLM | |
| 650 | 7 | |a Fluorescent Dyes |2 NLM | |
| 650 | 7 | |a Magnetite Nanoparticles |2 NLM | |
| 650 | 7 | |a Rhodamines |2 NLM | |
| 650 | 7 | |a graphene oxide |2 NLM | |
| 650 | 7 | |a tetramethylrhodamine |2 NLM | |
| 650 | 7 | |a 62669-72-1 |2 NLM | |
| 650 | 7 | |a Aflatoxin M1 |2 NLM | |
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| 650 | 7 | |a Cadaverine |2 NLM | |
| 650 | 7 | |a L90BEN6OLL |2 NLM | |
| 700 | 1 | |a Zhang, Xijie |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Lianjun |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Xianqing |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yu |e verfasserin |4 aut | |
| 700 | 1 | |a Qiao, Mingwu |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Wanjing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Tongtong |e verfasserin |4 aut | |
| 700 | 1 | |a Qi, Yuchen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Wuzhou |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Xuezhi |e verfasserin |4 aut | |
| 700 | 1 | |a Dou, Leina |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Huijuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Liye |e verfasserin |4 aut | |
| 700 | 1 | |a Mao, Yexuan |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Zhanhui |e verfasserin |4 aut | |
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| 856 | 4 | 0 | |u http://dx.doi.org/10.1007/s00604-021-04715-2 |3 Volltext |
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