Pretreatment with intestinal trefoil factor alleviates stress-induced gastric mucosal damage via Akt signaling
©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved..
BACKGROUND: Stress-related gastric mucosal damage or ulcer remains an unsolved issue for critically ill patients. Stress ulcer prophylaxis has been part of routine intensive care, but uncertainty and controversy still exist. Co-secreted with mucins, intestinal trefoil factor (ITF) is reported to promote restitution and regeneration of intestinal mucosal epithelium, although the mechanism remains unknown.
AIM: To elucidate the protective effects of ITF on gastric mucosa and explore the possible mechanisms.
METHODS: We used a rat model of gastric mucosal damage induced by water immersion restraint stress and lipopolysaccharide-treated human gastric epithelial cell line to investigate the potential effects of ITF on damaged gastric mucosa both in vivo and in vitro.
RESULTS: ITF promoted the proliferation and migration and inhibited necrosis of gastric mucosal epithelia in vitro. It also preserved the integrity of gastric mucosa by upregulating expressions of occludin and zonula occludens-1. In the rat model, pretreatment with ITF ameliorated the gastric mucosal epithelial damage and facilitated mucosal repair. The protective effects of ITF were confirmed to be exerted via activation of Akt signaling, and the specific inhibitor of Akt signaling LY249002 reversed the protective effects.
CONCLUSION: ITF might be a promising candidate for prevention and treatment of stress-induced gastric mucosal damage, and further studies should be undertaken to verify its clinical feasibility.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:26 |
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| Enthalten in: |
World journal of gastroenterology - 26(2020), 48 vom: 28. Dez., Seite 7619-7632 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Huang, Yun [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 14.05.2021 Date Revised 14.05.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.3748/wjg.v26.i48.7619 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM32066869X |
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| 100 | 1 | |a Huang, Yun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Pretreatment with intestinal trefoil factor alleviates stress-induced gastric mucosal damage via Akt signaling |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Revised 14.05.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a ©The Author(s) 2020. Published by Baishideng Publishing Group Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: Stress-related gastric mucosal damage or ulcer remains an unsolved issue for critically ill patients. Stress ulcer prophylaxis has been part of routine intensive care, but uncertainty and controversy still exist. Co-secreted with mucins, intestinal trefoil factor (ITF) is reported to promote restitution and regeneration of intestinal mucosal epithelium, although the mechanism remains unknown | ||
| 520 | |a AIM: To elucidate the protective effects of ITF on gastric mucosa and explore the possible mechanisms | ||
| 520 | |a METHODS: We used a rat model of gastric mucosal damage induced by water immersion restraint stress and lipopolysaccharide-treated human gastric epithelial cell line to investigate the potential effects of ITF on damaged gastric mucosa both in vivo and in vitro | ||
| 520 | |a RESULTS: ITF promoted the proliferation and migration and inhibited necrosis of gastric mucosal epithelia in vitro. It also preserved the integrity of gastric mucosa by upregulating expressions of occludin and zonula occludens-1. In the rat model, pretreatment with ITF ameliorated the gastric mucosal epithelial damage and facilitated mucosal repair. The protective effects of ITF were confirmed to be exerted via activation of Akt signaling, and the specific inhibitor of Akt signaling LY249002 reversed the protective effects | ||
| 520 | |a CONCLUSION: ITF might be a promising candidate for prevention and treatment of stress-induced gastric mucosal damage, and further studies should be undertaken to verify its clinical feasibility | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Akt signaling pathway | |
| 650 | 4 | |a Epithelium integrity | |
| 650 | 4 | |a Gastric mucosa | |
| 650 | 4 | |a Intestinal trefoil factor | |
| 650 | 4 | |a Water immersion restraint stress | |
| 650 | 7 | |a Growth Substances |2 NLM | |
| 650 | 7 | |a Muscle Proteins |2 NLM | |
| 650 | 7 | |a Neuropeptides |2 NLM | |
| 650 | 7 | |a Peptides |2 NLM | |
| 650 | 7 | |a Trefoil Factor-2 |2 NLM | |
| 650 | 7 | |a Trefoil Factor-3 |2 NLM | |
| 650 | 7 | |a Proto-Oncogene Proteins c-akt |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
| 700 | 1 | |a Wang, Meng-Meng |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Zhi-Zhou |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Zhao-Rui |e verfasserin |4 aut | |
| 700 | 1 | |a Nie, Shi-Nan |e verfasserin |4 aut | |
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