Binding of the SARS-CoV-2 spike protein to glycans
© 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved..
The pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a high number of deaths in the world. To combat it, it is necessary to develop a better understanding of how the virus infects host cells. Infection normally starts with the attachment of the virus to cell-surface glycans like heparan sulfate (HS) and sialic acid-containing glycolipids/glycoproteins. In this study, we examined and compared the binding of the subunits and spike (S) proteins of SARS-CoV-2, SARS-CoV, and Middle East respiratory disease (MERS)-CoV to these glycans. Our results revealed that the S proteins and subunits can bind to HS in a sulfation-dependent manner and no binding with sialic acid residues was detected. Overall, this work suggests that HS binding may be a general mechanism for the attachment of these coronaviruses to host cells, and supports the potential importance of HS in infection and in the development of antiviral agents against these viruses.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:66 |
|---|---|
| Enthalten in: |
Science bulletin - 66(2021), 12 vom: 30. Juni, Seite 1205-1214 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Hao, Wei [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Glycan microarray |
|---|
| Anmerkungen: |
Date Revised 19.09.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.1016/j.scib.2021.01.010 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM320577309 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM320577309 | ||
| 003 | DE-627 | ||
| 005 | 20231225173858.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.scib.2021.01.010 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1068.xml |
| 035 | |a (DE-627)NLM320577309 | ||
| 035 | |a (NLM)33495714 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Hao, Wei |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Binding of the SARS-CoV-2 spike protein to glycans |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 19.09.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2021 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved. | ||
| 520 | |a The pandemic of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a high number of deaths in the world. To combat it, it is necessary to develop a better understanding of how the virus infects host cells. Infection normally starts with the attachment of the virus to cell-surface glycans like heparan sulfate (HS) and sialic acid-containing glycolipids/glycoproteins. In this study, we examined and compared the binding of the subunits and spike (S) proteins of SARS-CoV-2, SARS-CoV, and Middle East respiratory disease (MERS)-CoV to these glycans. Our results revealed that the S proteins and subunits can bind to HS in a sulfation-dependent manner and no binding with sialic acid residues was detected. Overall, this work suggests that HS binding may be a general mechanism for the attachment of these coronaviruses to host cells, and supports the potential importance of HS in infection and in the development of antiviral agents against these viruses | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Glycan microarray | |
| 650 | 4 | |a Heparan sulfate | |
| 650 | 4 | |a SARS-CoV-2 S protein | |
| 650 | 4 | |a Sialic acid | |
| 650 | 4 | |a Sulfation | |
| 650 | 4 | |a Surface plasmon resonance | |
| 700 | 1 | |a Ma, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Ziheng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaoyu |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Xiaopan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yaohao |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Bo |e verfasserin |4 aut | |
| 700 | 1 | |a Shang, Shiying |e verfasserin |4 aut | |
| 700 | 1 | |a Cui, Sheng |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Zhongping |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Science bulletin |d 2015 |g 66(2021), 12 vom: 30. Juni, Seite 1205-1214 |w (DE-627)NLM249522675 |x 2095-9281 |7 nnns |
| 773 | 1 | 8 | |g volume:66 |g year:2021 |g number:12 |g day:30 |g month:06 |g pages:1205-1214 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.scib.2021.01.010 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 66 |j 2021 |e 12 |b 30 |c 06 |h 1205-1214 | ||