Runx2-Twist1 interaction coordinates cranial neural crest guidance of soft palate myogenesis
© 2021, Han et al..
Cranial neural crest (CNC) cells give rise to bone, cartilage, tendons, and ligaments of the vertebrate craniofacial musculoskeletal complex, as well as regulate mesoderm-derived craniofacial muscle development through cell-cell interactions. Using the mouse soft palate as a model, we performed an unbiased single-cell RNA-seq analysis to investigate the heterogeneity and lineage commitment of CNC derivatives during craniofacial muscle development. We show that Runx2, a known osteogenic regulator, is expressed in the CNC-derived perimysial and progenitor populations. Loss of Runx2 in CNC-derivatives results in reduced expression of perimysial markers (Aldh1a2 and Hic1) as well as soft palate muscle defects in Osr2-Cre;Runx2fl/fl mice. We further reveal that Runx2 maintains perimysial marker expression through suppressing Twist1, and that myogenesis is restored in Osr2-Cre;Runx2fl/fl;Twist1fl/+ mice. Collectively, our findings highlight the roles of Runx2, Twist1, and their interaction in regulating the fate of CNC-derived cells as they guide craniofacial muscle development through cell-cell interactions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
eLife - 10(2021) vom: 22. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Han, Xia [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 02.02.2022 Date Revised 02.02.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.7554/eLife.62387 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM320443116 |
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| 100 | 1 | |a Han, Xia |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Runx2-Twist1 interaction coordinates cranial neural crest guidance of soft palate myogenesis |
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| 520 | |a © 2021, Han et al. | ||
| 520 | |a Cranial neural crest (CNC) cells give rise to bone, cartilage, tendons, and ligaments of the vertebrate craniofacial musculoskeletal complex, as well as regulate mesoderm-derived craniofacial muscle development through cell-cell interactions. Using the mouse soft palate as a model, we performed an unbiased single-cell RNA-seq analysis to investigate the heterogeneity and lineage commitment of CNC derivatives during craniofacial muscle development. We show that Runx2, a known osteogenic regulator, is expressed in the CNC-derived perimysial and progenitor populations. Loss of Runx2 in CNC-derivatives results in reduced expression of perimysial markers (Aldh1a2 and Hic1) as well as soft palate muscle defects in Osr2-Cre;Runx2fl/fl mice. We further reveal that Runx2 maintains perimysial marker expression through suppressing Twist1, and that myogenesis is restored in Osr2-Cre;Runx2fl/fl;Twist1fl/+ mice. Collectively, our findings highlight the roles of Runx2, Twist1, and their interaction in regulating the fate of CNC-derived cells as they guide craniofacial muscle development through cell-cell interactions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Runx2 | |
| 650 | 4 | |a cell-cell interaction | |
| 650 | 4 | |a cleft palate | |
| 650 | 4 | |a cranial neural crest cells | |
| 650 | 4 | |a developmental biology | |
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| 650 | 4 | |a muscle development | |
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| 650 | 7 | |a Runx2 protein, mouse |2 NLM | |
| 650 | 7 | |a Twist-Related Protein 1 |2 NLM | |
| 650 | 7 | |a Twist1 protein, mouse |2 NLM | |
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| 700 | 1 | |a Yuan, Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Ho, Thach-Vu |e verfasserin |4 aut | |
| 700 | 1 | |a Cho, Courtney Kyeong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jingyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Jing, Junjun |e verfasserin |4 aut | |
| 700 | 1 | |a Janeckova, Eva |e verfasserin |4 aut | |
| 700 | 1 | |a He, Jinzhi |e verfasserin |4 aut | |
| 700 | 1 | |a Pei, Fei |e verfasserin |4 aut | |
| 700 | 1 | |a Bi, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Brian |e verfasserin |4 aut | |
| 700 | 1 | |a Chai, Yang |e verfasserin |4 aut | |
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