Renal AAV2-Mediated Overexpression of Long Non-Coding RNA H19 Attenuates Ischemic Acute Kidney Injury Through Sponging of microRNA-30a-5p
Copyright © 2021 by the American Society of Nephrology..
BACKGROUND: Renal ischemia-reperfusion (I/R) injury is a major cause of AKI. Noncoding RNAs are intricately involved in the pathophysiology of this form of AKI. Transcription of hypoxia-induced, long noncoding RNA H19, which shows high embryonic expression and is silenced in adults, is upregulated in renal I/R injury.
METHODS: Lentivirus-mediated overexpression, as well as antisense oligonucleotide-based silencing, modulated H19 in vitro. In vivo analyses used constitutive H19 knockout mice. In addition, renal vein injection of adeno-associated virus 2 (AAV2) carrying H19 caused overexpression in the kidney. Expression of H19 in kidney transplant patients with I/R injury was investigated.
RESULTS: H19 is upregulated in kidney biopsies of patients with AKI, in murine ischemic kidney tissue, and in cultured and ex vivo sorted hypoxic endothelial cells (ECs) and tubular epithelial cells (TECs). Transcription factors hypoxia-inducible factor 1-α, LHX8, and SPI1 activate H19 in ECs and TECs. H19 overexpression promotes angiogenesis in vitro and in vivo. In vivo, transient AAV2-mediated H19 overexpression significantly improved kidney function, reduced apoptosis, and reduced inflammation, as well as preserving capillary density and tubular epithelial integrity. Sponging of miR-30a-5p mediated the effects, which, in turn, led to target regulation of Dll4, ATG5, and Snai1.
CONCLUSIONS: H19 overexpression confers protection against renal injury by stimulating proangiogenic signaling. H19 overexpression may be a promising future therapeutic option in the treatment of patients with ischemic AKI.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:32 |
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| Enthalten in: |
Journal of the American Society of Nephrology : JASN - 32(2021), 2 vom: 21. Feb., Seite 323-341 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Haddad, George [VerfasserIn] |
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| Links: |
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| Themen: |
H19 |
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| Anmerkungen: |
Date Completed 23.08.2021 Date Revised 03.02.2023 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1681/ASN.2020060775 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM320412458 |
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| 100 | 1 | |a Haddad, George |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Renal AAV2-Mediated Overexpression of Long Non-Coding RNA H19 Attenuates Ischemic Acute Kidney Injury Through Sponging of microRNA-30a-5p |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Completed 23.08.2021 | ||
| 500 | |a Date Revised 03.02.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 by the American Society of Nephrology. | ||
| 520 | |a BACKGROUND: Renal ischemia-reperfusion (I/R) injury is a major cause of AKI. Noncoding RNAs are intricately involved in the pathophysiology of this form of AKI. Transcription of hypoxia-induced, long noncoding RNA H19, which shows high embryonic expression and is silenced in adults, is upregulated in renal I/R injury | ||
| 520 | |a METHODS: Lentivirus-mediated overexpression, as well as antisense oligonucleotide-based silencing, modulated H19 in vitro. In vivo analyses used constitutive H19 knockout mice. In addition, renal vein injection of adeno-associated virus 2 (AAV2) carrying H19 caused overexpression in the kidney. Expression of H19 in kidney transplant patients with I/R injury was investigated | ||
| 520 | |a RESULTS: H19 is upregulated in kidney biopsies of patients with AKI, in murine ischemic kidney tissue, and in cultured and ex vivo sorted hypoxic endothelial cells (ECs) and tubular epithelial cells (TECs). Transcription factors hypoxia-inducible factor 1-α, LHX8, and SPI1 activate H19 in ECs and TECs. H19 overexpression promotes angiogenesis in vitro and in vivo. In vivo, transient AAV2-mediated H19 overexpression significantly improved kidney function, reduced apoptosis, and reduced inflammation, as well as preserving capillary density and tubular epithelial integrity. Sponging of miR-30a-5p mediated the effects, which, in turn, led to target regulation of Dll4, ATG5, and Snai1 | ||
| 520 | |a CONCLUSIONS: H19 overexpression confers protection against renal injury by stimulating proangiogenic signaling. H19 overexpression may be a promising future therapeutic option in the treatment of patients with ischemic AKI | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a H19 | |
| 650 | 4 | |a ischemic acute kidney injury | |
| 650 | 4 | |a lncRNA | |
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| 700 | 1 | |a Kölling, Malte |e verfasserin |4 aut | |
| 700 | 1 | |a Wegmann, Urs A |e verfasserin |4 aut | |
| 700 | 1 | |a Dettling, Angela |e verfasserin |4 aut | |
| 700 | 1 | |a Seeger, Harald |e verfasserin |4 aut | |
| 700 | 1 | |a Schmitt, Roland |e verfasserin |4 aut | |
| 700 | 1 | |a Soerensen-Zender, Inga |e verfasserin |4 aut | |
| 700 | 1 | |a Haller, Hermann |e verfasserin |4 aut | |
| 700 | 1 | |a Kistler, Andreas D |e verfasserin |4 aut | |
| 700 | 1 | |a Dueck, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Engelhardt, Stefan |e verfasserin |4 aut | |
| 700 | 1 | |a Thum, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Mueller, Thomas F |e verfasserin |4 aut | |
| 700 | 1 | |a Wüthrich, Rudolf P |e verfasserin |4 aut | |
| 700 | 1 | |a Lorenzen, Johan M |e verfasserin |4 aut | |
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