Biomanufacturing of value-added products from oils or fats : A case study on cellular and fermentation engineering of Yarrowia lipolytica
© 2021 Wiley Periodicals LLC..
The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that converts waste oils or fats into a series of value-added products. Our research employs the oleaginous yeast Yarrowia lipolytica as a case study for citric acid (CA) production from waste oils. First, we conducted the computational fluid dynamics (CFD) simulation of the bioreactor system and identified that the extracellular mixing and mass transfer is the first limiting factor of an oil fermentation process due to the insolubility of oil in water. Based on the CFD simulation results, the bioreactor design and operating conditions were optimized and successfully enhanced oil uptake and bioconversion in fed-batch fermentation experiments. After that, we investigated the impacts of cell morphology on oil uptake, intracellular lipid accumulation, and CA formation by overexpressing and deleting the MHY1 gene in the wild type Y. lipolytica ATCC20362. Fairly good linear correlations (R2 > 0.82) were achieved between cell morphology and productivities of biomass, lipid, and CA. Finally, fermentation kinetics with both glucose and oil substrates were compared and the oil fermentation process was carefully evaluated. Our study suggests that waste oils or fats can be economical feedstocks for biomanufacturing of many high-value products.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:118 |
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| Enthalten in: |
Biotechnology and bioengineering - 118(2021), 4 vom: 15. Apr., Seite 1677-1692 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Na [VerfasserIn] |
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| Links: |
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| Themen: |
2968PHW8QP |
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| Anmerkungen: |
Date Completed 17.01.2022 Date Revised 17.01.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/bit.27685 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM320328236 |
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| 245 | 1 | 0 | |a Biomanufacturing of value-added products from oils or fats |b A case study on cellular and fermentation engineering of Yarrowia lipolytica |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 Wiley Periodicals LLC. | ||
| 520 | |a The United States produces more than 10 million tons of waste oils and fats each year. This paper aims to establish a new biomanufacturing platform that converts waste oils or fats into a series of value-added products. Our research employs the oleaginous yeast Yarrowia lipolytica as a case study for citric acid (CA) production from waste oils. First, we conducted the computational fluid dynamics (CFD) simulation of the bioreactor system and identified that the extracellular mixing and mass transfer is the first limiting factor of an oil fermentation process due to the insolubility of oil in water. Based on the CFD simulation results, the bioreactor design and operating conditions were optimized and successfully enhanced oil uptake and bioconversion in fed-batch fermentation experiments. After that, we investigated the impacts of cell morphology on oil uptake, intracellular lipid accumulation, and CA formation by overexpressing and deleting the MHY1 gene in the wild type Y. lipolytica ATCC20362. Fairly good linear correlations (R2 > 0.82) were achieved between cell morphology and productivities of biomass, lipid, and CA. Finally, fermentation kinetics with both glucose and oil substrates were compared and the oil fermentation process was carefully evaluated. Our study suggests that waste oils or fats can be economical feedstocks for biomanufacturing of many high-value products | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a CFD simulation | |
| 650 | 4 | |a Y. lipolytica | |
| 650 | 4 | |a biomanufacturing | |
| 650 | 4 | |a cell morphology | |
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| 700 | 1 | |a Mirzaee, Iman |e verfasserin |4 aut | |
| 700 | 1 | |a Olsen, Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Hsi-Wu |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Dongming |e verfasserin |4 aut | |
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