Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations
© 2021, Johnson et al..
Laboratory experimental evolution provides a window into the details of the evolutionary process. To investigate the consequences of long-term adaptation, we evolved 205 Saccharomyces cerevisiae populations (124 haploid and 81 diploid) for ~10,000,000 generations in three environments. We measured the dynamics of fitness changes over time, finding repeatable patterns of declining adaptability. Sequencing revealed that this phenotypic adaptation is coupled with a steady accumulation of mutations, widespread genetic parallelism, and historical contingency. In contrast to long-term evolution in E. coli, we do not observe long-term coexistence or populations with highly elevated mutation rates. We find that evolution in diploid populations involves both fixation of heterozygous mutations and frequent loss-of-heterozygosity events. Together, these results help distinguish aspects of evolutionary dynamics that are likely to be general features of adaptation across many systems from those that are specific to individual organisms and environmental conditions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
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| Enthalten in: |
eLife - 10(2021) vom: 19. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Johnson, Milo S [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 28.01.2022 Date Revised 30.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.7554/eLife.63910 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populations |
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| 520 | |a © 2021, Johnson et al. | ||
| 520 | |a Laboratory experimental evolution provides a window into the details of the evolutionary process. To investigate the consequences of long-term adaptation, we evolved 205 Saccharomyces cerevisiae populations (124 haploid and 81 diploid) for ~10,000,000 generations in three environments. We measured the dynamics of fitness changes over time, finding repeatable patterns of declining adaptability. Sequencing revealed that this phenotypic adaptation is coupled with a steady accumulation of mutations, widespread genetic parallelism, and historical contingency. In contrast to long-term evolution in E. coli, we do not observe long-term coexistence or populations with highly elevated mutation rates. We find that evolution in diploid populations involves both fixation of heterozygous mutations and frequent loss-of-heterozygosity events. Together, these results help distinguish aspects of evolutionary dynamics that are likely to be general features of adaptation across many systems from those that are specific to individual organisms and environmental conditions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 4 | |a S. cerevisiae | |
| 650 | 4 | |a dynamics of adaptation | |
| 650 | 4 | |a evolutionary biology | |
| 650 | 4 | |a experimental evolution | |
| 650 | 4 | |a yeast | |
| 700 | 1 | |a Gopalakrishnan, Shreyas |e verfasserin |4 aut | |
| 700 | 1 | |a Goyal, Juhee |e verfasserin |4 aut | |
| 700 | 1 | |a Dillingham, Megan E |e verfasserin |4 aut | |
| 700 | 1 | |a Bakerlee, Christopher W |e verfasserin |4 aut | |
| 700 | 1 | |a Humphrey, Parris T |e verfasserin |4 aut | |
| 700 | 1 | |a Jagdish, Tanush |e verfasserin |4 aut | |
| 700 | 1 | |a Jerison, Elizabeth R |e verfasserin |4 aut | |
| 700 | 1 | |a Kosheleva, Katya |e verfasserin |4 aut | |
| 700 | 1 | |a Lawrence, Katherine R |e verfasserin |4 aut | |
| 700 | 1 | |a Min, Jiseon |e verfasserin |4 aut | |
| 700 | 1 | |a Moulana, Alief |e verfasserin |4 aut | |
| 700 | 1 | |a Phillips, Angela M |e verfasserin |4 aut | |
| 700 | 1 | |a Piper, Julia C |e verfasserin |4 aut | |
| 700 | 1 | |a Purkanti, Ramya |e verfasserin |4 aut | |
| 700 | 1 | |a Rego-Costa, Artur |e verfasserin |4 aut | |
| 700 | 1 | |a McDonald, Michael J |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen Ba, Alex N |e verfasserin |4 aut | |
| 700 | 1 | |a Desai, Michael M |e verfasserin |4 aut | |
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