Colon cancer-associated transcript-1 enhances glucose metabolism and colon cancer cell activity in a high-glucose environment in vitro and in vivo
2020 Journal of Gastrointestinal Oncology. All rights reserved..
BACKGROUND: Our study aims to investigate the effect of colon cancer-associated transcript-1 (CCAT-1) on colon cancer cells' activity and metabolism under different glucose environments in vitro and in vivo.
METHODS: The levels of proliferation, migration, glucose, lactic acid, glucose metabolism-related enzymes, apoptosis genes, epithelial-mesenchymal transition (EMT) marker proteins, and PI3K/Akt/C-MYC pathway in CCAT-1-silenced SW620 cells cultured with different glucose levels were tested. Twenty BALB/C nude mice with hyperglycemia or normal blood sugar were transplanted with CCAT-1-silenced SW620 cells, blood glucose levels, lactic acid, insulin, and volume of transplanted tumor cells, the expression of EMT marker proteins, and PI3K/Akt/C-MYC pathway was detected.
RESULTS: The levels of proliferation, migration, glucose, lactic acid, LDH-A, PKM2, and HK2 decreased, apoptosis increased in SW620 cells cultured with low glucose or silenced CCAT-1 (P<0.05); levels of E-cadherin and ZO-1 significantly increased, and levels of N-cadherin, vimentin, and p-Akt decreased in CCAT-1-silenced SW620 cells cultured with high glucose (P<0.05). Hyperglycemic nude mice transplanted with CCAT-1-silenced colon cancer cells showed decreased tumor volume, blood glucose, lactic acid, insulin, P-AKT, and P-C-MYC than EV group (P<0.05).
CONCLUSIONS: CCAT-1 can enhance glucose metabolism and proliferation and migration of colon cancer cells by upregulating the expression of glycolysis enzymes, inhibiting apoptosis, activating the Akt/C-MYC pathway, and promoting EMT expression.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Journal of gastrointestinal oncology - 11(2020), 6 vom: 30. Dez., Seite 1164-1185 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cui, Ge [VerfasserIn] |
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| Links: |
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| Themen: |
C-MYC |
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| Anmerkungen: |
Date Revised 19.04.2022 published: Print Citation Status PubMed-not-MEDLINE |
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| doi: |
10.21037/jgo-20-474 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM320196070 |
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| 100 | 1 | |a Cui, Ge |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Colon cancer-associated transcript-1 enhances glucose metabolism and colon cancer cell activity in a high-glucose environment in vitro and in vivo |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a 2020 Journal of Gastrointestinal Oncology. All rights reserved. | ||
| 520 | |a BACKGROUND: Our study aims to investigate the effect of colon cancer-associated transcript-1 (CCAT-1) on colon cancer cells' activity and metabolism under different glucose environments in vitro and in vivo | ||
| 520 | |a METHODS: The levels of proliferation, migration, glucose, lactic acid, glucose metabolism-related enzymes, apoptosis genes, epithelial-mesenchymal transition (EMT) marker proteins, and PI3K/Akt/C-MYC pathway in CCAT-1-silenced SW620 cells cultured with different glucose levels were tested. Twenty BALB/C nude mice with hyperglycemia or normal blood sugar were transplanted with CCAT-1-silenced SW620 cells, blood glucose levels, lactic acid, insulin, and volume of transplanted tumor cells, the expression of EMT marker proteins, and PI3K/Akt/C-MYC pathway was detected | ||
| 520 | |a RESULTS: The levels of proliferation, migration, glucose, lactic acid, LDH-A, PKM2, and HK2 decreased, apoptosis increased in SW620 cells cultured with low glucose or silenced CCAT-1 (P<0.05); levels of E-cadherin and ZO-1 significantly increased, and levels of N-cadherin, vimentin, and p-Akt decreased in CCAT-1-silenced SW620 cells cultured with high glucose (P<0.05). Hyperglycemic nude mice transplanted with CCAT-1-silenced colon cancer cells showed decreased tumor volume, blood glucose, lactic acid, insulin, P-AKT, and P-C-MYC than EV group (P<0.05) | ||
| 520 | |a CONCLUSIONS: CCAT-1 can enhance glucose metabolism and proliferation and migration of colon cancer cells by upregulating the expression of glycolysis enzymes, inhibiting apoptosis, activating the Akt/C-MYC pathway, and promoting EMT expression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a C-MYC | |
| 650 | 4 | |a Colon cancer-related transcript-1 (CCAT-1) | |
| 650 | 4 | |a PI3K signaling pathway | |
| 650 | 4 | |a colon cancer | |
| 650 | 4 | |a glucose metabolism | |
| 650 | 4 | |a hyperglycemia | |
| 700 | 1 | |a Huang, Yuxuan |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Wenming |e verfasserin |4 aut | |
| 700 | 1 | |a Yao, Yunliang |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Hongchang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xining |e verfasserin |4 aut | |
| 700 | 1 | |a Gong, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Yifan |e verfasserin |4 aut | |
| 700 | 1 | |a Sun, Yandi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Mengya |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Yan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Ting |e verfasserin |4 aut | |
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