Cell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo
Recently, studies about RNA modification dynamics in human RNAs are among the most controversially discussed. As a main reason, we identified the unavailability of a technique which allows the investigation of the temporal processing of RNA transcripts. Here, we present nucleic acid isotope labeling coupled mass spectrometry (NAIL-MS) for efficient, monoisotopic stable isotope labeling in both RNA and DNA in standard cell culture. We design pulse chase experiments and study the temporal placement of modified nucleosides in tRNAPhe and 18S rRNA. In existing RNAs, we observe a time-dependent constant loss of modified nucleosides which is masked by post-transcriptional methylation mechanisms and thus undetectable without NAIL-MS. During alkylation stress, NAIL-MS reveals an adaptation of tRNA modifications in new transcripts but not existing ones. Overall, we present a fast and reliable stable isotope labeling strategy which allows in-depth study of RNA modification dynamics in human cell culture.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Nature communications - 12(2021), 1 vom: 15. Jan., Seite 389 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Heiss, Matthias [VerfasserIn] |
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| Links: |
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| Themen: |
Evaluation Study |
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| Anmerkungen: |
Date Completed 29.01.2021 Date Revised 30.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-020-20576-4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Recently, studies about RNA modification dynamics in human RNAs are among the most controversially discussed. As a main reason, we identified the unavailability of a technique which allows the investigation of the temporal processing of RNA transcripts. Here, we present nucleic acid isotope labeling coupled mass spectrometry (NAIL-MS) for efficient, monoisotopic stable isotope labeling in both RNA and DNA in standard cell culture. We design pulse chase experiments and study the temporal placement of modified nucleosides in tRNAPhe and 18S rRNA. In existing RNAs, we observe a time-dependent constant loss of modified nucleosides which is masked by post-transcriptional methylation mechanisms and thus undetectable without NAIL-MS. During alkylation stress, NAIL-MS reveals an adaptation of tRNA modifications in new transcripts but not existing ones. Overall, we present a fast and reliable stable isotope labeling strategy which allows in-depth study of RNA modification dynamics in human cell culture | ||
| 650 | 4 | |a Evaluation Study | |
| 650 | 4 | |a Journal Article | |
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| 700 | 1 | |a Hagelskamp, Felix |e verfasserin |4 aut | |
| 700 | 1 | |a Marchand, Virginie |e verfasserin |4 aut | |
| 700 | 1 | |a Motorin, Yuri |e verfasserin |4 aut | |
| 700 | 1 | |a Kellner, Stefanie |e verfasserin |4 aut | |
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