Conformational Plasticity of Cyclic Ras-Inhibitor Peptides Defines Cell Permeabilization Activity
© 2021 Wiley-VCH GmbH..
Cyclorasins 9A5 and 9A54 are 11-mer cyclic peptides that inhibit the Ras-Raf protein interaction. The peptides share a cell-penetrating peptide (CPP)-like motif; however, only cyclorasin 9A5 can permeabilize cells to exhibit strong cell-based activity. To unveil the structural origin underlying their distinct cellular permeabilization activities, we compared the three-dimensional structures of cyclorasins 9A5 and 9A54 in water and in the less polar solvent dimethyl sulfoxide (DMSO) by solution NMR. We found that cyclorasin 9A5 changes its extended conformation in water to a compact amphipathic structure with converged aromatic residues surrounded by Arg residues in DMSO, which might contribute to its cell permeabilization activity. However, cyclorasin 9A54 cannot adopt this amphipathic structure, due to the steric hindrance between two neighboring bulky amino-acid sidechains, Tle-2 and dVal-3. We also found that the bulkiness of the sidechains at positions 2 and 3 negatively affects the cell permeabilization activities, indicating that the conformational plasticity that allows the peptides to form the amphipathic structure is important for their cell permeabilization activities.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:60 |
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| Enthalten in: |
Angewandte Chemie (International ed. in English) - 60(2021), 12 vom: 15. März, Seite 6567-6572 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Takeuchi, Koh [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 14.07.2021 Date Revised 14.07.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/anie.202016647 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © 2021 Wiley-VCH GmbH. | ||
| 520 | |a Cyclorasins 9A5 and 9A54 are 11-mer cyclic peptides that inhibit the Ras-Raf protein interaction. The peptides share a cell-penetrating peptide (CPP)-like motif; however, only cyclorasin 9A5 can permeabilize cells to exhibit strong cell-based activity. To unveil the structural origin underlying their distinct cellular permeabilization activities, we compared the three-dimensional structures of cyclorasins 9A5 and 9A54 in water and in the less polar solvent dimethyl sulfoxide (DMSO) by solution NMR. We found that cyclorasin 9A5 changes its extended conformation in water to a compact amphipathic structure with converged aromatic residues surrounded by Arg residues in DMSO, which might contribute to its cell permeabilization activity. However, cyclorasin 9A54 cannot adopt this amphipathic structure, due to the steric hindrance between two neighboring bulky amino-acid sidechains, Tle-2 and dVal-3. We also found that the bulkiness of the sidechains at positions 2 and 3 negatively affects the cell permeabilization activities, indicating that the conformational plasticity that allows the peptides to form the amphipathic structure is important for their cell permeabilization activities | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a NMR spectroscopy | |
| 650 | 4 | |a cell permeabilization | |
| 650 | 4 | |a peptides | |
| 650 | 4 | |a protein-protein interaction inhibitor | |
| 650 | 4 | |a structure | |
| 650 | 7 | |a Peptides, Cyclic |2 NLM | |
| 650 | 7 | |a raf Kinases |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
| 650 | 7 | |a ras Proteins |2 NLM | |
| 650 | 7 | |a EC 3.6.5.2 |2 NLM | |
| 700 | 1 | |a Misaki, Imai |e verfasserin |4 aut | |
| 700 | 1 | |a Tokunaga, Yuji |e verfasserin |4 aut | |
| 700 | 1 | |a Fujisaki, Miwa |e verfasserin |4 aut | |
| 700 | 1 | |a Kamoshida, Hajime |e verfasserin |4 aut | |
| 700 | 1 | |a Takizawa, Takeshi |e verfasserin |4 aut | |
| 700 | 1 | |a Hanzawa, Hiroyuki |e verfasserin |4 aut | |
| 700 | 1 | |a Shimada, Ichio |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:60 |g year:2021 |g number:12 |g day:15 |g month:03 |g pages:6567-6572 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/anie.202016647 |3 Volltext |
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