Protamine 1/Protamine 2 mRNA ratio in nonobstructive azoospermic patients
© 2021 Wiley-VCH GmbH..
Altered protamine 1 (PRM1)/ protamine 2 (PRM2) mRNA ratio in testicular biopsy samples correlates with sperm quality and its fertilising ability. This study is planned to assess PRM1/ PRM2 mRNA ratio in subgroups of azoospermia to suggest a more reliable and accurate marker for assessing sperm quality in nonobstructive azoospermia (NOA). A cross-sectional study was done on testicular biopsy samples, taken from 106 azoospermic patients. Samples were histologically classified into subgroups: 36 obstructive azoospermia (OA), and two groups of NOA: 41 round spermatid maturation arrest (SMA) and 29 Sertoli cell-only syndrome (SCOS). OA samples showed histologically normal spermatogenesis and serve as a positive control. mRNA expression of jumonji domain-containing 1A (JMJD1A), PRM1, PRM2 and transition nuclear proteins (TNP1, TNP2) genes was determined, by RT-qPCR. Significantly lower expression of JMJD1A (p < .001), PRM1 (p = .0265) and PRM2 (p = .0032) has been seen in the SCOS group of NOA. We found significant (p < .001) increase in PRM1/PRM2 mRNA ratio in testicular biopsy samples of SCOS group of NOA patients and significant negative correlation of PRM1/PRM2 mRNA ratio with JMJD1A. Hence, PRM1/PRM2 mRNA ratio may represent a more reliable and accurate marker to assess sperm quality in NOA in addition to standard semen parameters.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Andrologia - 53(2021), 3 vom: 01. Apr., Seite e13936 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Amjad, Sofia [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.06.2021 Date Revised 31.05.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/and.13936 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM319908275 |
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| 520 | |a © 2021 Wiley-VCH GmbH. | ||
| 520 | |a Altered protamine 1 (PRM1)/ protamine 2 (PRM2) mRNA ratio in testicular biopsy samples correlates with sperm quality and its fertilising ability. This study is planned to assess PRM1/ PRM2 mRNA ratio in subgroups of azoospermia to suggest a more reliable and accurate marker for assessing sperm quality in nonobstructive azoospermia (NOA). A cross-sectional study was done on testicular biopsy samples, taken from 106 azoospermic patients. Samples were histologically classified into subgroups: 36 obstructive azoospermia (OA), and two groups of NOA: 41 round spermatid maturation arrest (SMA) and 29 Sertoli cell-only syndrome (SCOS). OA samples showed histologically normal spermatogenesis and serve as a positive control. mRNA expression of jumonji domain-containing 1A (JMJD1A), PRM1, PRM2 and transition nuclear proteins (TNP1, TNP2) genes was determined, by RT-qPCR. Significantly lower expression of JMJD1A (p < .001), PRM1 (p = .0265) and PRM2 (p = .0032) has been seen in the SCOS group of NOA. We found significant (p < .001) increase in PRM1/PRM2 mRNA ratio in testicular biopsy samples of SCOS group of NOA patients and significant negative correlation of PRM1/PRM2 mRNA ratio with JMJD1A. Hence, PRM1/PRM2 mRNA ratio may represent a more reliable and accurate marker to assess sperm quality in NOA in addition to standard semen parameters | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Sertoli cell-only syndrome | |
| 650 | 4 | |a azoospermia | |
| 650 | 4 | |a nonobstructive azoospermia | |
| 650 | 4 | |a protamine 1/protamine 2 mRNA ratio | |
| 650 | 4 | |a round spermatid maturation arrest | |
| 650 | 7 | |a PRM1 protein, human |2 NLM | |
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| 700 | 1 | |a Mushtaq, Shamim |e verfasserin |4 aut | |
| 700 | 1 | |a Rehman, Rehana |e verfasserin |4 aut | |
| 700 | 1 | |a Munir, Adnan |e verfasserin |4 aut | |
| 700 | 1 | |a Zahid, Nida |e verfasserin |4 aut | |
| 700 | 1 | |a Siddique, Pirzada Qasim Raza |e verfasserin |4 aut | |
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