RNA-Seq identifies genes whose proteins are upregulated during syncytia development in murine C2C12 myoblasts and human BeWo trophoblasts
© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society..
The fusion of villous cytotrophoblasts into the multinucleated syncytiotrophoblast is critical for the essential functions of the mammalian placenta. Using RNA-Seq gene expression, quantitative protein expression, and siRNA knockdown we identified genes and their cognate proteins which are similarly upregulated in two cellular models of mammalian syncytia development (human BeWo cytotrophoblast to syncytiotrophoblast and murine C2C12 myoblast to myotube). These include DYSF, PDE4DIP, SPIRE2, NDRG1, PLEC, GPR146, HSPB8, DHCR7, and HDAC5. These findings provide avenues for further understanding of the mechanisms underlying mammalian placental syncytiotrophoblast development.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:9 |
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| Enthalten in: |
Physiological reports - 9(2021), 1 vom: 01. Jan., Seite e14671 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Azar, Christopher [VerfasserIn] |
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| Links: |
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| Themen: |
Cell fusion |
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| Anmerkungen: |
Date Completed 21.12.2021 Date Revised 01.04.2024 published: Print Citation Status MEDLINE |
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| doi: |
10.14814/phy2.14671 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a © 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society. | ||
| 520 | |a The fusion of villous cytotrophoblasts into the multinucleated syncytiotrophoblast is critical for the essential functions of the mammalian placenta. Using RNA-Seq gene expression, quantitative protein expression, and siRNA knockdown we identified genes and their cognate proteins which are similarly upregulated in two cellular models of mammalian syncytia development (human BeWo cytotrophoblast to syncytiotrophoblast and murine C2C12 myoblast to myotube). These include DYSF, PDE4DIP, SPIRE2, NDRG1, PLEC, GPR146, HSPB8, DHCR7, and HDAC5. These findings provide avenues for further understanding of the mechanisms underlying mammalian placental syncytiotrophoblast development | ||
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| 700 | 1 | |a Rois, Lisa |e verfasserin |4 aut | |
| 700 | 1 | |a Mahjoub, Moe |e verfasserin |4 aut | |
| 700 | 1 | |a Nelson, D Michael |e verfasserin |4 aut | |
| 700 | 1 | |a Schwartz, Alan L |e verfasserin |4 aut | |
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