SARS-CoV-2 escape in vitro from a highly neutralizing COVID-19 convalescent plasma
To investigate the evolution of SARS-CoV-2 in the immune population, we co-incubated authentic virus with a highly neutralizing plasma from a COVID-19 convalescent patient. The plasma fully neutralized the virus for 7 passages, but after 45 days, the deletion of F140 in the spike N-terminal domain (NTD) N3 loop led to partial breakthrough. At day 73, an E484K substitution in the receptor-binding domain (RBD) occurred, followed at day 80 by an insertion in the NTD N5 loop containing a new glycan sequon, which generated a variant completely resistant to plasma neutralization. Computational modeling predicts that the deletion and insertion in loops N3 and N5 prevent binding of neutralizing antibodies. The recent emergence in the United Kingdom and South Africa of natural variants with similar changes suggests that SARS-CoV-2 has the potential to escape an effective immune response and that vaccines and antibodies able to control emerging variants should be developed.
ONE SENTENCE SUMMARY: Three mutations allowed SARS-CoV-2 to evade the polyclonal antibody response of a highly neutralizing COVID-19 convalescent plasma.
Errataetall: |
UpdateIn: Proc Natl Acad Sci U S A. 2021 Sep 7;118(36):. - PMID 34417349 |
---|---|
Medienart: |
E-Artikel |
Erscheinungsjahr: |
2020 |
---|---|
Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - year:2020 |
---|---|
Enthalten in: |
bioRxiv : the preprint server for biology - (2020) vom: 28. Dez. |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Andreano, Emanuele [VerfasserIn] |
---|
Links: |
---|
Themen: |
---|
Anmerkungen: |
Date Revised 30.03.2024 published: Electronic UpdateIn: Proc Natl Acad Sci U S A. 2021 Sep 7;118(36):. - PMID 34417349 Citation Status PubMed-not-MEDLINE |
---|
doi: |
10.1101/2020.12.28.424451 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM31962188X |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | NLM31962188X | ||
003 | DE-627 | ||
005 | 20240330235117.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1101/2020.12.28.424451 |2 doi | |
028 | 5 | 2 | |a pubmed24n1356.xml |
035 | |a (DE-627)NLM31962188X | ||
035 | |a (NLM)33398278 | ||
035 | |a (PII)2020.12.28.424451 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Andreano, Emanuele |e verfasserin |4 aut | |
245 | 1 | 0 | |a SARS-CoV-2 escape in vitro from a highly neutralizing COVID-19 convalescent plasma |
264 | 1 | |c 2020 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Revised 30.03.2024 | ||
500 | |a published: Electronic | ||
500 | |a UpdateIn: Proc Natl Acad Sci U S A. 2021 Sep 7;118(36):. - PMID 34417349 | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a To investigate the evolution of SARS-CoV-2 in the immune population, we co-incubated authentic virus with a highly neutralizing plasma from a COVID-19 convalescent patient. The plasma fully neutralized the virus for 7 passages, but after 45 days, the deletion of F140 in the spike N-terminal domain (NTD) N3 loop led to partial breakthrough. At day 73, an E484K substitution in the receptor-binding domain (RBD) occurred, followed at day 80 by an insertion in the NTD N5 loop containing a new glycan sequon, which generated a variant completely resistant to plasma neutralization. Computational modeling predicts that the deletion and insertion in loops N3 and N5 prevent binding of neutralizing antibodies. The recent emergence in the United Kingdom and South Africa of natural variants with similar changes suggests that SARS-CoV-2 has the potential to escape an effective immune response and that vaccines and antibodies able to control emerging variants should be developed | ||
520 | |a ONE SENTENCE SUMMARY: Three mutations allowed SARS-CoV-2 to evade the polyclonal antibody response of a highly neutralizing COVID-19 convalescent plasma | ||
650 | 4 | |a Preprint | |
700 | 1 | |a Piccini, Giulia |e verfasserin |4 aut | |
700 | 1 | |a Licastro, Danilo |e verfasserin |4 aut | |
700 | 1 | |a Casalino, Lorenzo |e verfasserin |4 aut | |
700 | 1 | |a Johnson, Nicole V |e verfasserin |4 aut | |
700 | 1 | |a Paciello, Ida |e verfasserin |4 aut | |
700 | 1 | |a Dal Monego, Simeone |e verfasserin |4 aut | |
700 | 1 | |a Pantano, Elisa |e verfasserin |4 aut | |
700 | 1 | |a Manganaro, Noemi |e verfasserin |4 aut | |
700 | 1 | |a Manenti, Alessandro |e verfasserin |4 aut | |
700 | 1 | |a Manna, Rachele |e verfasserin |4 aut | |
700 | 1 | |a Casa, Elisa |e verfasserin |4 aut | |
700 | 1 | |a Hyseni, Inesa |e verfasserin |4 aut | |
700 | 1 | |a Benincasa, Linda |e verfasserin |4 aut | |
700 | 1 | |a Montomoli, Emanuele |e verfasserin |4 aut | |
700 | 1 | |a Amaro, Rommie E |e verfasserin |4 aut | |
700 | 1 | |a McLellan, Jason S |e verfasserin |4 aut | |
700 | 1 | |a Rappuoli, Rino |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t bioRxiv : the preprint server for biology |d 2020 |g (2020) vom: 28. Dez. |w (DE-627)NLM31090014X |7 nnns |
773 | 1 | 8 | |g year:2020 |g day:28 |g month:12 |
856 | 4 | 0 | |u http://dx.doi.org/10.1101/2020.12.28.424451 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |j 2020 |b 28 |c 12 |