Lung cancer cell-derived EDA-containing fibronectin induces an inflammatory response from monocytes and promotes metastatic tumor microenvironment
© 2020 Wiley Periodicals LLC..
Tumor-associated macrophages (TAMs) play a pivotal role in facilitating tumor growth and metastasis. This tumor-promoting propensity of TAMs sets in as a result of their complex cross-talk with tumor cells mediated primarily by tumor cell-secreted proteins in the tumor microenvironment. To explore such interactions, we employed an immunoscreening approach involving the immunization of Balb-c mice with model human lung carcinoma cell line, A549. From serological examination combined with mass spectrometric analysis, EDA-containing fibronectin (EDAFN ) was identified as a conspicuous immunogenic protein in A549 cell secretome. We showed that A549 secreted EDAFN engages TLR-4 on THP-1 monocytes to drive the proinflammatory response via NF-κB signaling cascade. Conversely, A549 derived EDAFN potentiates their metastatic capacity by inducing epithelial-mesenchymal transition through its autocrine activity. In conclusion, the study proposes a possible mechanism of cellular cross-talk between lung cancer cells and associated monocytes mediated by lung cancer-derived EDAFN and resulting in the establishment of proinflammatory and metastatic tumor microenvironment.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:122 |
|---|---|
| Enthalten in: |
Journal of cellular biochemistry - 122(2021), 5 vom: 01. Mai, Seite 562-576 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Amin, Asif [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Fibronectin |
|---|
| Anmerkungen: |
Date Completed 04.10.2021 Date Revised 04.10.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/jcb.29883 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM319571343 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM319571343 | ||
| 003 | DE-627 | ||
| 005 | 20231225171732.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/jcb.29883 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1065.xml |
| 035 | |a (DE-627)NLM319571343 | ||
| 035 | |a (NLM)33393138 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Amin, Asif |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Lung cancer cell-derived EDA-containing fibronectin induces an inflammatory response from monocytes and promotes metastatic tumor microenvironment |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 04.10.2021 | ||
| 500 | |a Date Revised 04.10.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Wiley Periodicals LLC. | ||
| 520 | |a Tumor-associated macrophages (TAMs) play a pivotal role in facilitating tumor growth and metastasis. This tumor-promoting propensity of TAMs sets in as a result of their complex cross-talk with tumor cells mediated primarily by tumor cell-secreted proteins in the tumor microenvironment. To explore such interactions, we employed an immunoscreening approach involving the immunization of Balb-c mice with model human lung carcinoma cell line, A549. From serological examination combined with mass spectrometric analysis, EDA-containing fibronectin (EDAFN ) was identified as a conspicuous immunogenic protein in A549 cell secretome. We showed that A549 secreted EDAFN engages TLR-4 on THP-1 monocytes to drive the proinflammatory response via NF-κB signaling cascade. Conversely, A549 derived EDAFN potentiates their metastatic capacity by inducing epithelial-mesenchymal transition through its autocrine activity. In conclusion, the study proposes a possible mechanism of cellular cross-talk between lung cancer cells and associated monocytes mediated by lung cancer-derived EDAFN and resulting in the establishment of proinflammatory and metastatic tumor microenvironment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a fibronectin | |
| 650 | 4 | |a inflammation | |
| 650 | 4 | |a metastasis | |
| 650 | 4 | |a secretome | |
| 650 | 4 | |a tumor microenvironment | |
| 650 | 7 | |a Fibronectins |2 NLM | |
| 700 | 1 | |a Mokhdomi, Taseem A |e verfasserin |4 aut | |
| 700 | 1 | |a Bukhari, Shoiab |e verfasserin |4 aut | |
| 700 | 1 | |a Wani, Zubair |e verfasserin |4 aut | |
| 700 | 1 | |a Chikan, Naveed A |e verfasserin |4 aut | |
| 700 | 1 | |a Shah, Basit A |e verfasserin |4 aut | |
| 700 | 1 | |a Koul, Aabid M |e verfasserin |4 aut | |
| 700 | 1 | |a Majeed, Umer |e verfasserin |4 aut | |
| 700 | 1 | |a Farooq, Faizah |e verfasserin |4 aut | |
| 700 | 1 | |a Qadri, Ayub |e verfasserin |4 aut | |
| 700 | 1 | |a Qadri, Raies A |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of cellular biochemistry |d 1985 |g 122(2021), 5 vom: 01. Mai, Seite 562-576 |w (DE-627)NLM012621285 |x 1097-4644 |7 nnns |
| 773 | 1 | 8 | |g volume:122 |g year:2021 |g number:5 |g day:01 |g month:05 |g pages:562-576 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/jcb.29883 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 122 |j 2021 |e 5 |b 01 |c 05 |h 562-576 | ||