Dexamethasone inhibits SARS-CoV-2 spike pseudotyped virus viropexis by binding to ACE2
Copyright © 2020 Elsevier Inc. All rights reserved..
The SARS-CoV-2 outbreak, began in late 2019, has caused a worldwide pandemic and shows no signs of slowing. Glucocorticoids (GCs), including dexamethasone (DEX), have been widely used as effective anti-inflammatory and immunosuppressant drugs. In this study, seven GCs had no obvious effect on cell viability of angiotensin converting enzyme 2 (ACE2) high expressed HEK293T cells when concentrations were under 10 μM. Molecular docking results revealed that DEX occupied with active binding site of ACE2 of SARS-CoV-2 spike protein. Surface plasmon resonance (SPR) results showed that KD value between DEX and ACE2 was (9.03 ± 0.78) e-6 M. Cell membrane chromatography (CMC) results uncovered that DEX had a chromatographic retention. DEX was found out to inhibiting the viropexis into ACE2h cells using SARS-CoV-2 spike pseudotyped virus. Therefore, DEX inhibits the entrance of SARS-CoV-2 spike pseudotyped virus into cell by binding to ACE2.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2021 |
---|---|
Erschienen: |
2021 |
Enthalten in: |
Zur Gesamtaufnahme - volume:554 |
---|---|
Enthalten in: |
Virology - 554(2021) vom: 15. Feb., Seite 83-88 |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Zhang, Yongjing [VerfasserIn] |
---|
Links: |
---|
Anmerkungen: |
Date Completed 28.01.2021 Date Revised 19.08.2022 published: Print-Electronic Citation Status MEDLINE |
---|
doi: |
10.1016/j.virol.2020.12.001 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM319518086 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM319518086 | ||
003 | DE-627 | ||
005 | 20231225171624.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2021 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.virol.2020.12.001 |2 doi | |
028 | 5 | 2 | |a pubmed24n1065.xml |
035 | |a (DE-627)NLM319518086 | ||
035 | |a (NLM)33387788 | ||
035 | |a (PII)S0042-6822(20)30240-3 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Zhang, Yongjing |e verfasserin |4 aut | |
245 | 1 | 0 | |a Dexamethasone inhibits SARS-CoV-2 spike pseudotyped virus viropexis by binding to ACE2 |
264 | 1 | |c 2021 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Completed 28.01.2021 | ||
500 | |a Date Revised 19.08.2022 | ||
500 | |a published: Print-Electronic | ||
500 | |a Citation Status MEDLINE | ||
520 | |a Copyright © 2020 Elsevier Inc. All rights reserved. | ||
520 | |a The SARS-CoV-2 outbreak, began in late 2019, has caused a worldwide pandemic and shows no signs of slowing. Glucocorticoids (GCs), including dexamethasone (DEX), have been widely used as effective anti-inflammatory and immunosuppressant drugs. In this study, seven GCs had no obvious effect on cell viability of angiotensin converting enzyme 2 (ACE2) high expressed HEK293T cells when concentrations were under 10 μM. Molecular docking results revealed that DEX occupied with active binding site of ACE2 of SARS-CoV-2 spike protein. Surface plasmon resonance (SPR) results showed that KD value between DEX and ACE2 was (9.03 ± 0.78) e-6 M. Cell membrane chromatography (CMC) results uncovered that DEX had a chromatographic retention. DEX was found out to inhibiting the viropexis into ACE2h cells using SARS-CoV-2 spike pseudotyped virus. Therefore, DEX inhibits the entrance of SARS-CoV-2 spike pseudotyped virus into cell by binding to ACE2 | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Research Support, Non-U.S. Gov't | |
650 | 4 | |a ACE2 | |
650 | 4 | |a Dexamethasone | |
650 | 4 | |a SARS-CoV-2 | |
650 | 7 | |a Spike Glycoprotein, Coronavirus |2 NLM | |
650 | 7 | |a spike protein, SARS-CoV-2 |2 NLM | |
650 | 7 | |a Dexamethasone |2 NLM | |
650 | 7 | |a 7S5I7G3JQL |2 NLM | |
650 | 7 | |a ACE2 protein, human |2 NLM | |
650 | 7 | |a EC 3.4.17.23 |2 NLM | |
650 | 7 | |a Angiotensin-Converting Enzyme 2 |2 NLM | |
650 | 7 | |a EC 3.4.17.23 |2 NLM | |
700 | 1 | |a Hu, Shiling |e verfasserin |4 aut | |
700 | 1 | |a Wang, Jue |e verfasserin |4 aut | |
700 | 1 | |a Xue, Zhuoyin |e verfasserin |4 aut | |
700 | 1 | |a Wang, Cheng |e verfasserin |4 aut | |
700 | 1 | |a Wang, Nan |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Virology |d 1955 |g 554(2021) vom: 15. Feb., Seite 83-88 |w (DE-627)NLM000011347 |x 1096-0341 |7 nnns |
773 | 1 | 8 | |g volume:554 |g year:2021 |g day:15 |g month:02 |g pages:83-88 |
856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.virol.2020.12.001 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 554 |j 2021 |b 15 |c 02 |h 83-88 |