Masseter response to long-term experimentally induced anterior crossbite in Sprague-Dawley rats
Copyright © 2020. Published by Elsevier Ltd..
OBJECTIVE: To detect the long-term response to unilateral anterior crossbite (UAC) in masticatory muscles and in molecular biomarkers of peripheral blood leukocytes.
DESIGN: Fifty-six six-week-old Sprague-Dawley rats were used. The gene-fold changes in peripheral blood leukocytes were detected by the microarray analysis to compare the rats that received 20-week UAC treatment with age-matched controls (n = 4). Muscle atrophy-related gene Fbxo32 was selected based on the data of the microarray analysis verified by using real-time PCR. The remaining 36 rats were randomly separated in the UAC and control groups at 12 and 20 weeks (n = 12). The protein expression of Fbxo32 and the muscle injury and myogenesis-related markers, αB-crystallin and desmin, were detected in the masseter and lateral pterygoid muscles by western blot assay.
RESULTS: In the 20-week UAC group, the masseter muscle weight was lower than that in the age-matched control group, and the expression level of Fbxo32 gene in peripheral blood leukocytes was increased according to the microarray analysis confirmed by real-time PCR detection. The increased protein expression levels of Fbxo32 were detected in the masseter in the 20-week UAC group, and the protein expression levels of desmin and αB-crystallin were decreased at this time point. No similar changes were detected in the lateral pterygoid muscle.
CONCLUSIONS: Masseter atrophy is induced by long-term stimulation of UAC. The increased expression of the Fbxo32 gene in peripheral blood leukocytes may be a candidate biological marker of masseter atrophy.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:122 |
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| Enthalten in: |
Archives of oral biology - 122(2021) vom: 05. Feb., Seite 104985 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Hong-Yun [VerfasserIn] |
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| Links: |
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| Themen: |
Atrophy |
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| Anmerkungen: |
Date Completed 12.02.2021 Date Revised 12.02.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.archoralbio.2020.104985 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM319057720 |
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| 100 | 1 | |a Zhang, Hong-Yun |e verfasserin |4 aut | |
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| 500 | |a Date Revised 12.02.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020. Published by Elsevier Ltd. | ||
| 520 | |a OBJECTIVE: To detect the long-term response to unilateral anterior crossbite (UAC) in masticatory muscles and in molecular biomarkers of peripheral blood leukocytes | ||
| 520 | |a DESIGN: Fifty-six six-week-old Sprague-Dawley rats were used. The gene-fold changes in peripheral blood leukocytes were detected by the microarray analysis to compare the rats that received 20-week UAC treatment with age-matched controls (n = 4). Muscle atrophy-related gene Fbxo32 was selected based on the data of the microarray analysis verified by using real-time PCR. The remaining 36 rats were randomly separated in the UAC and control groups at 12 and 20 weeks (n = 12). The protein expression of Fbxo32 and the muscle injury and myogenesis-related markers, αB-crystallin and desmin, were detected in the masseter and lateral pterygoid muscles by western blot assay | ||
| 520 | |a RESULTS: In the 20-week UAC group, the masseter muscle weight was lower than that in the age-matched control group, and the expression level of Fbxo32 gene in peripheral blood leukocytes was increased according to the microarray analysis confirmed by real-time PCR detection. The increased protein expression levels of Fbxo32 were detected in the masseter in the 20-week UAC group, and the protein expression levels of desmin and αB-crystallin were decreased at this time point. No similar changes were detected in the lateral pterygoid muscle | ||
| 520 | |a CONCLUSIONS: Masseter atrophy is induced by long-term stimulation of UAC. The increased expression of the Fbxo32 gene in peripheral blood leukocytes may be a candidate biological marker of masseter atrophy | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Atrophy | |
| 650 | 4 | |a Fbxo32 | |
| 650 | 4 | |a Masticatory muscles | |
| 650 | 4 | |a Occlusion | |
| 650 | 4 | |a Peripheral blood leukocytes | |
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| 650 | 7 | |a Fbxo32 protein, rat |2 NLM | |
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| 650 | 7 | |a SKP Cullin F-Box Protein Ligases |2 NLM | |
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| 700 | 1 | |a Duan, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Mian-Jiao |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jin-Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Hong-Xu |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Mei-Qing |e verfasserin |4 aut | |
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