Modeling Burn Progression Using Comb Burns : The Impact of Thermal Contact Duration on Model Outcomes
Copyright © 2020 Elsevier Inc. All rights reserved..
BACKGROUND: Burn progression is a phenomenon that remains poorly characterized. The mechanisms of burn conversion are not completely understood, and consequently, both predictive diagnostic tools and interventions are limited. The rat comb burn model is a commonly used approach to study horizontal burn conversion. However, there is significant variability in how the model is performed. Skin contact duration, comb device heating method, comb heating duration, amount of pressure applied, the weight of the comb, and associated depth of burn are all variables that are heterogeneous in studies utilizing the model.
MATERIALS AND METHODS: Here, contact duration was examined to determine the impact the duration of burn delivery has on the conversion of interspaces in this model. Data from multiple experiments consisting of 10, 15, 20, 30, 40, and 45 s comb burns were compiled and assessed. Burns were made using combs heated in a 100°C dry bath and then monitored for 2 d. Interspace viability was assessed by digital and laser doppler imaging and biopsy procurement.
RESULTS: Laser Doppler Imaging and viable interspace measurements showed that as burn duration increased, the percentage of the viable interspace and interspace perfusion decreased. Additionally, a contact time of 30 s or greater was required to result in 100% interspace conversion.
CONCLUSIONS: These results demonstrate a need to better characterize and potentially standardize the rat comb burn model to reduce variation and maintain it as a valuable tool for controlled studies of the pathophysiology of burn wound progression.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:260 |
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| Enthalten in: |
The Journal of surgical research - 260(2021) vom: 25. Apr., Seite 155-162 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Smith, Robert D [VerfasserIn] |
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| Links: |
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| Themen: |
Burn depth |
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| Anmerkungen: |
Date Completed 10.09.2021 Date Revised 10.09.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jss.2020.11.068 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM319057224 |
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| 245 | 1 | 0 | |a Modeling Burn Progression Using Comb Burns |b The Impact of Thermal Contact Duration on Model Outcomes |
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| 500 | |a Date Completed 10.09.2021 | ||
| 500 | |a Date Revised 10.09.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: Burn progression is a phenomenon that remains poorly characterized. The mechanisms of burn conversion are not completely understood, and consequently, both predictive diagnostic tools and interventions are limited. The rat comb burn model is a commonly used approach to study horizontal burn conversion. However, there is significant variability in how the model is performed. Skin contact duration, comb device heating method, comb heating duration, amount of pressure applied, the weight of the comb, and associated depth of burn are all variables that are heterogeneous in studies utilizing the model | ||
| 520 | |a MATERIALS AND METHODS: Here, contact duration was examined to determine the impact the duration of burn delivery has on the conversion of interspaces in this model. Data from multiple experiments consisting of 10, 15, 20, 30, 40, and 45 s comb burns were compiled and assessed. Burns were made using combs heated in a 100°C dry bath and then monitored for 2 d. Interspace viability was assessed by digital and laser doppler imaging and biopsy procurement | ||
| 520 | |a RESULTS: Laser Doppler Imaging and viable interspace measurements showed that as burn duration increased, the percentage of the viable interspace and interspace perfusion decreased. Additionally, a contact time of 30 s or greater was required to result in 100% interspace conversion | ||
| 520 | |a CONCLUSIONS: These results demonstrate a need to better characterize and potentially standardize the rat comb burn model to reduce variation and maintain it as a valuable tool for controlled studies of the pathophysiology of burn wound progression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Burn depth | |
| 650 | 4 | |a Burn progression | |
| 650 | 4 | |a Comb burn model | |
| 650 | 4 | |a Laser Doppler imaging | |
| 700 | 1 | |a Carney, Bonnie C |e verfasserin |4 aut | |
| 700 | 1 | |a Garg, Gaurav |e verfasserin |4 aut | |
| 700 | 1 | |a Monger, Kyle W |e verfasserin |4 aut | |
| 700 | 1 | |a Prindeze, Nicholas J |e verfasserin |4 aut | |
| 700 | 1 | |a Shupp, Jeffrey W |e verfasserin |4 aut | |
| 700 | 1 | |a Moffatt, Lauren T |e verfasserin |4 aut | |
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