Expression of Cathepsins B, D, and G in Microcystic Lymphatic Malformation
Background: This study investigated the expression and localization of cathepsins B, D, and G in relationship to the embryonic stem cell (ESC)-like population we have previously identified in microcystic lymphatic malformation (mLM). Methods and Results: Immunohistochemical staining demonstrated expression of cathepsins B, D, and G in cervicofacial mLM tissue samples from 11 patients. Immunofluorescence staining of two representative mLM samples showed localization of cathepsins B and D to the OCT4+ and the c-MYC+ cells on the endothelium of lesional vessels and the stroma, while cathepsin G was localized to the OCT4+/tryptase+ cells within the stroma. Transcript expression of cathepsins B, D, and G was confirmed using reverse transcription quantitative polymerase chain reaction (RT-qPCR; n = 5). Western blotting (n = 3) performed on the mLM tissue samples revealed protein expression of cathepsins B and D, which were demonstrated to be enzymatically active using enzymatic activity assays. Conclusion: This study demonstrated expression of cathepsins B and D by the ESC-like cells on the endothelium of lesional vessels and the stroma, while cathepsin G was localized to the OCT4+ phenotypic mast cells within the stroma of mLM.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:19 |
|---|---|
| Enthalten in: |
Lymphatic research and biology - 19(2021), 4 vom: 13. Aug., Seite 347-354 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Mehrotra, Shreeja [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 17.09.2021 Date Revised 31.05.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1089/lrb.2020.0047 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM31902850X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM31902850X | ||
| 003 | DE-627 | ||
| 005 | 20231225170605.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1089/lrb.2020.0047 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1063.xml |
| 035 | |a (DE-627)NLM31902850X | ||
| 035 | |a (NLM)33337924 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Mehrotra, Shreeja |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Expression of Cathepsins B, D, and G in Microcystic Lymphatic Malformation |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 17.09.2021 | ||
| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Background: This study investigated the expression and localization of cathepsins B, D, and G in relationship to the embryonic stem cell (ESC)-like population we have previously identified in microcystic lymphatic malformation (mLM). Methods and Results: Immunohistochemical staining demonstrated expression of cathepsins B, D, and G in cervicofacial mLM tissue samples from 11 patients. Immunofluorescence staining of two representative mLM samples showed localization of cathepsins B and D to the OCT4+ and the c-MYC+ cells on the endothelium of lesional vessels and the stroma, while cathepsin G was localized to the OCT4+/tryptase+ cells within the stroma. Transcript expression of cathepsins B, D, and G was confirmed using reverse transcription quantitative polymerase chain reaction (RT-qPCR; n = 5). Western blotting (n = 3) performed on the mLM tissue samples revealed protein expression of cathepsins B and D, which were demonstrated to be enzymatically active using enzymatic activity assays. Conclusion: This study demonstrated expression of cathepsins B and D by the ESC-like cells on the endothelium of lesional vessels and the stroma, while cathepsin G was localized to the OCT4+ phenotypic mast cells within the stroma of mLM | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a cathepsin B | |
| 650 | 4 | |a cathepsin D | |
| 650 | 4 | |a cathepsin G | |
| 650 | 4 | |a embryonic stem cells | |
| 650 | 4 | |a lymphatic malformation | |
| 650 | 4 | |a microcystic | |
| 650 | 4 | |a renin–angiotensin system | |
| 650 | 7 | |a CTSG protein, human |2 NLM | |
| 650 | 7 | |a EC 3.4.21.20 |2 NLM | |
| 650 | 7 | |a Cathepsin G |2 NLM | |
| 650 | 7 | |a EC 3.4.21.20 |2 NLM | |
| 650 | 7 | |a CTSB protein, human |2 NLM | |
| 650 | 7 | |a EC 3.4.22.1 |2 NLM | |
| 650 | 7 | |a Cathepsin B |2 NLM | |
| 650 | 7 | |a EC 3.4.22.1 |2 NLM | |
| 650 | 7 | |a CTSD protein, human |2 NLM | |
| 650 | 7 | |a EC 3.4.23.5 |2 NLM | |
| 650 | 7 | |a Cathepsin D |2 NLM | |
| 650 | 7 | |a EC 3.4.23.5 |2 NLM | |
| 700 | 1 | |a van Schaijik, Bede |e verfasserin |4 aut | |
| 700 | 1 | |a Boyes, Kendra |e verfasserin |4 aut | |
| 700 | 1 | |a Bockett, Nicholas |e verfasserin |4 aut | |
| 700 | 1 | |a Brasch, Helen D |e verfasserin |4 aut | |
| 700 | 1 | |a Davis, Paul F |e verfasserin |4 aut | |
| 700 | 1 | |a Itinteang, Tinte |e verfasserin |4 aut | |
| 700 | 1 | |a Tan, Swee T |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Lymphatic research and biology |d 2003 |g 19(2021), 4 vom: 13. Aug., Seite 347-354 |w (DE-627)NLM152728295 |x 1557-8585 |7 nnns |
| 773 | 1 | 8 | |g volume:19 |g year:2021 |g number:4 |g day:13 |g month:08 |g pages:347-354 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1089/lrb.2020.0047 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 19 |j 2021 |e 4 |b 13 |c 08 |h 347-354 | ||