IGI-LuNER : single-well multiplexed RT-qPCR test for SARS-CoV-2
Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a sample control in a single, multiplexed reaction. We developed the IGI-LuNER RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (NER). This combined, cost-effective test can be performed in 384-well plates with detection sensitivity suitable for clinical reporting, and will aid in future sample pooling efforts, thus improving throughput of SARS-CoV-2 detection.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2020 |
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| Enthalten in: |
medRxiv : the preprint server for health sciences - (2020) vom: 11. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Stahl, Elizabeth C [VerfasserIn] |
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| Links: |
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| Themen: |
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| Anmerkungen: |
Date Revised 19.10.2023 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1101/2020.12.10.20247338 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM318959356 |
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| 500 | |a published: Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Commonly used RT-qPCR-based SARS-CoV-2 diagnostics require 2-3 separate reactions or rely on detection of a single viral target, adding time and cost or risk of false-negative results. Currently, no test combines detection of widely used SARS-CoV-2 E- and N-gene targets and a sample control in a single, multiplexed reaction. We developed the IGI-LuNER RT-qPCR assay using the Luna Probe Universal One-Step RT-qPCR master mix with publicly available primers and probes to detect SARS-CoV-2 N gene, E gene, and human RNase P (NER). This combined, cost-effective test can be performed in 384-well plates with detection sensitivity suitable for clinical reporting, and will aid in future sample pooling efforts, thus improving throughput of SARS-CoV-2 detection | ||
| 650 | 4 | |a Preprint | |
| 700 | 1 | |a Tsuchida, Connor A |e verfasserin |4 aut | |
| 700 | 1 | |a Hamilton, Jennifer R |e verfasserin |4 aut | |
| 700 | 1 | |a Lin-Shiao, Enrique |e verfasserin |4 aut | |
| 700 | 1 | |a McDevitt, Shana L |e verfasserin |4 aut | |
| 700 | 1 | |a Moehle, Erica A |e verfasserin |4 aut | |
| 700 | 1 | |a Witkowsky, Lea B |e verfasserin |4 aut | |
| 700 | 1 | |a Tsui, C Kimberly |e verfasserin |4 aut | |
| 700 | 1 | |a Pestal, Kathleen |e verfasserin |4 aut | |
| 700 | 1 | |a Gildea, Holly K |e verfasserin |4 aut | |
| 700 | 1 | |a McElroy, Matthew |e verfasserin |4 aut | |
| 700 | 1 | |a Keller, Amanda |e verfasserin |4 aut | |
| 700 | 1 | |a Sylvain, Iman |e verfasserin |4 aut | |
| 700 | 1 | |a Williams, Clara |e verfasserin |4 aut | |
| 700 | 1 | |a Hirsh, Ariana |e verfasserin |4 aut | |
| 700 | 1 | |a Ciling, Alison |e verfasserin |4 aut | |
| 700 | 1 | |a Ehrenberg, Alexander J |e verfasserin |4 aut | |
| 700 | 0 | |a IGI SARS-CoV-2 consortium |e verfasserin |4 aut | |
| 700 | 1 | |a Urnov, Fyodor D |e verfasserin |4 aut | |
| 700 | 1 | |a Ringeisen, Bradley R |e verfasserin |4 aut | |
| 700 | 1 | |a Giannikopoulos, Petros |e verfasserin |4 aut | |
| 700 | 1 | |a Doudna, Jennifer A |e verfasserin |4 aut | |
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