Mitochondrial misreading in skeletal muscle accelerates metabolic aging and confers lipid accumulation and increased inflammation
Published by Cold Spring Harbor Laboratory Press for the RNA Society..
We have recently reported on an experimental model of mitochondrial mistranslation conferred by amino acid exchange V338Y in the mitochondrial ribosomal protein MrpS5. Here we used a combination of RNA-Seq and metabolic profiling of homozygous transgenic MrpS5V338Y/V338Y mice to analyze the changes associated with the V338Y mutation in post-mitotic skeletal muscle. Metabolic profiling demonstrated age-dependent metabolic changes in the mutant V338Y animals, which included enhanced levels of age-associated metabolites and which were accompanied by increased glycolysis, lipid desaturation and eicosanoid biosynthesis, and alterations of the pentose phosphate pathway. In addition, transcriptome signatures of aged V338Y mutant muscle pointed to elevated inflammation, likely reflecting the increased levels of bioactive lipids. Our findings indicate that mistranslation-mediated chronic impairment of mitochondrial function affects specific bioenergetic processes in muscle in an age-dependent manner.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2020 |
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| Enthalten in: |
RNA (New York, N.Y.) - (2020) vom: 01. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Scherbakov, Dimitri [VerfasserIn] |
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| Links: |
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| Themen: |
Aging |
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| Anmerkungen: |
Date Revised 22.02.2024 published: Print-Electronic Citation Status Publisher |
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| doi: |
10.1261/rna.077347.120 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM318284790 |
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| 100 | 1 | |a Scherbakov, Dimitri |c Sr |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Mitochondrial misreading in skeletal muscle accelerates metabolic aging and confers lipid accumulation and increased inflammation |
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| 500 | |a Date Revised 22.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status Publisher | ||
| 520 | |a Published by Cold Spring Harbor Laboratory Press for the RNA Society. | ||
| 520 | |a We have recently reported on an experimental model of mitochondrial mistranslation conferred by amino acid exchange V338Y in the mitochondrial ribosomal protein MrpS5. Here we used a combination of RNA-Seq and metabolic profiling of homozygous transgenic MrpS5V338Y/V338Y mice to analyze the changes associated with the V338Y mutation in post-mitotic skeletal muscle. Metabolic profiling demonstrated age-dependent metabolic changes in the mutant V338Y animals, which included enhanced levels of age-associated metabolites and which were accompanied by increased glycolysis, lipid desaturation and eicosanoid biosynthesis, and alterations of the pentose phosphate pathway. In addition, transcriptome signatures of aged V338Y mutant muscle pointed to elevated inflammation, likely reflecting the increased levels of bioactive lipids. Our findings indicate that mistranslation-mediated chronic impairment of mitochondrial function affects specific bioenergetic processes in muscle in an age-dependent manner | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Aging | |
| 650 | 4 | |a Metabolome | |
| 650 | 4 | |a Misreading | |
| 650 | 4 | |a Mitochondria | |
| 650 | 4 | |a Skeletal Muscle | |
| 700 | 1 | |a Duscha, Stefan |c Jr |e verfasserin |4 aut | |
| 700 | 1 | |a Juskeviciene, Reda |c 2nd |e verfasserin |4 aut | |
| 700 | 1 | |a Restelli, Lisa |c 3rd |e verfasserin |4 aut | |
| 700 | 1 | |a Frank, Stephan |c 4th |e verfasserin |4 aut | |
| 700 | 1 | |a Laczko, Endre |c 5th |e verfasserin |4 aut | |
| 700 | 1 | |a Boettger, Erik C |c 6th |e verfasserin |4 aut | |
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