The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple-negative breast cancer cells
© 2020 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies..
The ubiquitin ligase RNF8 is known to induce epithelial-to-mesenchymal (EMT) transition and metastasis in triple-negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis. In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell motility. We find that RNF8 knockdown in TNBC cells attenuates the protein and mRNA levels of Ras homolog family member A (RHOA) and cell division cycle 42 (CDC42). We show that the formation of filopodia, focal adhesions, and the association of focal adhesions to stress fibers is impaired upon RNF8 knockdown. Cell migration is significantly inhibited by RNF8 knockdown. Our study suggests a potential novel role for RNF8 in mediating cell migration in TNBC through regulation of the Rho GTPases RHOA and CDC42.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:595 |
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| Enthalten in: |
FEBS letters - 595(2021), 2 vom: 01. Jan., Seite 241-252 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Pereira De Carvalho, Bruno [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.07.2021 Date Revised 15.07.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/1873-3468.13999 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317726447 |
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| 100 | 1 | |a Pereira De Carvalho, Bruno |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The ubiquitin ligase RNF8 regulates Rho GTPases and promotes cytoskeletal changes and motility in triple-negative breast cancer cells |
| 264 | 1 | |c 2021 | |
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| 500 | |a Date Revised 15.07.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 The Authors. FEBS Letters published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. | ||
| 520 | |a The ubiquitin ligase RNF8 is known to induce epithelial-to-mesenchymal (EMT) transition and metastasis in triple-negative breast cancer (TNBC). Besides EMT, Rho GTPases have been shown as key regulators in metastasis. In this study, we investigated the role of RNF8 in regulating Rho GTPases and cell motility. We find that RNF8 knockdown in TNBC cells attenuates the protein and mRNA levels of Ras homolog family member A (RHOA) and cell division cycle 42 (CDC42). We show that the formation of filopodia, focal adhesions, and the association of focal adhesions to stress fibers is impaired upon RNF8 knockdown. Cell migration is significantly inhibited by RNF8 knockdown. Our study suggests a potential novel role for RNF8 in mediating cell migration in TNBC through regulation of the Rho GTPases RHOA and CDC42 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a CDC42 | |
| 650 | 4 | |a RHOA | |
| 650 | 4 | |a RNF8 | |
| 650 | 4 | |a cell migration | |
| 650 | 4 | |a cytoskeleton rearrangements | |
| 650 | 4 | |a filopodia | |
| 650 | 4 | |a focal adhesion | |
| 650 | 4 | |a triple-negative breast cancer | |
| 650 | 7 | |a DNA-Binding Proteins |2 NLM | |
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| 700 | 1 | |a Chern, Yi-Jye |e verfasserin |4 aut | |
| 700 | 1 | |a He, Jiabei |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Chia-Hsin |e verfasserin |4 aut | |
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