Gain-of-function assay for SARS-CoV-2 M pro inhibition in living cells
The main protease, M pro , of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M pro function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing.
| Errataetall: | |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - year:2020 |
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| Enthalten in: |
bioRxiv : the preprint server for biology - (2020) vom: 09. Nov. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Moghadasi, Seyad Arad [VerfasserIn] |
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| Anmerkungen: |
Date Revised 03.05.2023 published: Electronic UpdateIn: mBio. 2022 Jun 28;13(3):e0078422. - PMID 35471084 Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1101/2020.11.09.375139 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317674382 |
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| 500 | |a UpdateIn: mBio. 2022 Jun 28;13(3):e0078422. - PMID 35471084 | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a The main protease, M pro , of SARS-CoV-2 is required to cleave the viral polyprotein into precise functional units for virus replication and pathogenesis. Here we demonstrate a quantitative reporter for M pro function in living cells, in which protease inhibition by genetic or chemical methods results in strong eGFP fluorescence. This robust gain-of-function system readily distinguishes between inhibitor potencies and can be scaled-up to high-throughput platforms for drug testing | ||
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| 700 | 1 | |a Belica, Christopher |e verfasserin |4 aut | |
| 700 | 1 | |a Wick, Chloe |e verfasserin |4 aut | |
| 700 | 1 | |a Brown, William L |e verfasserin |4 aut | |
| 700 | 1 | |a Harris, Reuben S |e verfasserin |4 aut | |
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