A comparison between the structures of reconstituted salivary pellicles and oral mucin (MUC5B) films
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved..
HYPOTHESIS: Salivary pellicles i.e., thin films formed upon selective adsorption of saliva, protect oral surfaces against chemical and mechanical insults. Pellicles are also excellent aqueous lubricants. It is generally accepted that reconstituted pellicles have a two-layer structure, where the outer layer is mainly composed of MUC5B mucins. We hypothesized that by comparing the effect of ionic strength on reconstituted pellicles and MUC5B films we could gain further insight into the pellicle structure.
EXPERIMENTS: Salivary pellicles and MUC5B films reconstituted on solid surfaces were investigated at different ionic strengths by Force Spectroscopy, Quartz Crystal Microbalance with Dissipation, Null Ellipsometry and Neutron Reflectometry.
FINDINGS: Our results support the two-layer structure for reconstituted salivary pellicles. The outer layer swelled when ionic strength decreased, indicating a weak polyelectrolyte behavior. While initially the MUC5B films exhibited a similar tendency, this was followed by a drastic collapse indicating an interaction between exposed hydrophobic domains. This suggests that mucins in the pellicle outer layer form complexes with other salivary components that prevent this interaction. Lowering ionic strength below physiological values also led to a partial removal of the pellicle inner layer. Overall, our results highlight the importance that the interactions of mucins with other pellicle components play on their structure.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:584 |
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| Enthalten in: |
Journal of colloid and interface science - 584(2021) vom: 15. Feb., Seite 660-668 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Boyd, Hannah [VerfasserIn] |
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| Links: |
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| Themen: |
Ionic strength |
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| Anmerkungen: |
Date Completed 21.06.2021 Date Revised 21.06.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jcis.2020.10.124 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 100 | 1 | |a Boyd, Hannah |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A comparison between the structures of reconstituted salivary pellicles and oral mucin (MUC5B) films |
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| 500 | |a Date Revised 21.06.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a HYPOTHESIS: Salivary pellicles i.e., thin films formed upon selective adsorption of saliva, protect oral surfaces against chemical and mechanical insults. Pellicles are also excellent aqueous lubricants. It is generally accepted that reconstituted pellicles have a two-layer structure, where the outer layer is mainly composed of MUC5B mucins. We hypothesized that by comparing the effect of ionic strength on reconstituted pellicles and MUC5B films we could gain further insight into the pellicle structure | ||
| 520 | |a EXPERIMENTS: Salivary pellicles and MUC5B films reconstituted on solid surfaces were investigated at different ionic strengths by Force Spectroscopy, Quartz Crystal Microbalance with Dissipation, Null Ellipsometry and Neutron Reflectometry | ||
| 520 | |a FINDINGS: Our results support the two-layer structure for reconstituted salivary pellicles. The outer layer swelled when ionic strength decreased, indicating a weak polyelectrolyte behavior. While initially the MUC5B films exhibited a similar tendency, this was followed by a drastic collapse indicating an interaction between exposed hydrophobic domains. This suggests that mucins in the pellicle outer layer form complexes with other salivary components that prevent this interaction. Lowering ionic strength below physiological values also led to a partial removal of the pellicle inner layer. Overall, our results highlight the importance that the interactions of mucins with other pellicle components play on their structure | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Ionic strength | |
| 650 | 4 | |a MUC5B | |
| 650 | 4 | |a Mucin | |
| 650 | 4 | |a Salivary pellicle | |
| 650 | 4 | |a Steric forces | |
| 650 | 7 | |a Mucin-5B |2 NLM | |
| 650 | 7 | |a Mucins |2 NLM | |
| 700 | 1 | |a Gonzalez-Martinez, Juan F |e verfasserin |4 aut | |
| 700 | 1 | |a Welbourn, Rebecca J L |e verfasserin |4 aut | |
| 700 | 1 | |a Gutfreund, Philipp |e verfasserin |4 aut | |
| 700 | 1 | |a Klechikov, Alexey |e verfasserin |4 aut | |
| 700 | 1 | |a Robertsson, Carolina |e verfasserin |4 aut | |
| 700 | 1 | |a Wickström, Claes |e verfasserin |4 aut | |
| 700 | 1 | |a Arnebrant, Thomas |e verfasserin |4 aut | |
| 700 | 1 | |a Barker, Robert |e verfasserin |4 aut | |
| 700 | 1 | |a Sotres, Javier |e verfasserin |4 aut | |
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