Fungal-bacterial interaction selects for quorum sensing mutants with increased production of natural antifungal compounds
Soil microorganisms coexist and interact showing antagonistic or mutualistic behaviors. Here, we show that an environmental strain of Bacillus subtilis undergoes heritable phenotypic variation upon interaction with the soil fungal pathogen Setophoma terrestris (ST). Metabolomics analysis revealed differential profiles in B. subtilis before (pre-ST) and after (post-ST) interacting with the fungus, which paradoxically involved the absence of lipopeptides surfactin and plipastatin and yet acquisition of antifungal activity in post-ST variants. The profile of volatile compounds showed that 2-heptanone and 2-octanone were the most discriminating metabolites present at higher concentrations in post-ST during the interaction process. Both ketones showed strong antifungal activity, which was lost with the addition of exogenous surfactin. Whole-genome analyses indicate that mutations in ComQPXA quorum-sensing system, constituted the genetic bases of post-ST conversion, which rewired B. subtilis metabolism towards the depletion of surfactins and the production of antifungal compounds during its antagonistic interaction with S. terrestris.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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| Enthalten in: |
Communications biology - 3(2020), 1 vom: 12. Nov., Seite 670 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Albarracín Orio, Andrea G [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 28.06.2021 Date Revised 31.03.2024 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s42003-020-01342-0 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317518968 |
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| 100 | 1 | |a Albarracín Orio, Andrea G |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Fungal-bacterial interaction selects for quorum sensing mutants with increased production of natural antifungal compounds |
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| 500 | |a Date Revised 31.03.2024 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Soil microorganisms coexist and interact showing antagonistic or mutualistic behaviors. Here, we show that an environmental strain of Bacillus subtilis undergoes heritable phenotypic variation upon interaction with the soil fungal pathogen Setophoma terrestris (ST). Metabolomics analysis revealed differential profiles in B. subtilis before (pre-ST) and after (post-ST) interacting with the fungus, which paradoxically involved the absence of lipopeptides surfactin and plipastatin and yet acquisition of antifungal activity in post-ST variants. The profile of volatile compounds showed that 2-heptanone and 2-octanone were the most discriminating metabolites present at higher concentrations in post-ST during the interaction process. Both ketones showed strong antifungal activity, which was lost with the addition of exogenous surfactin. Whole-genome analyses indicate that mutations in ComQPXA quorum-sensing system, constituted the genetic bases of post-ST conversion, which rewired B. subtilis metabolism towards the depletion of surfactins and the production of antifungal compounds during its antagonistic interaction with S. terrestris | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 7 | |a Antifungal Agents |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 650 | 7 | |a Biological Products |2 NLM | |
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| 700 | 1 | |a Petras, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Tobares, Romina A |e verfasserin |4 aut | |
| 700 | 1 | |a Aksenov, Alexander A |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Mingxun |e verfasserin |4 aut | |
| 700 | 1 | |a Juncosa, Florencia |e verfasserin |4 aut | |
| 700 | 1 | |a Sayago, Pamela |e verfasserin |4 aut | |
| 700 | 1 | |a Moyano, Alejandro J |e verfasserin |4 aut | |
| 700 | 1 | |a Dorrestein, Pieter C |e verfasserin |4 aut | |
| 700 | 1 | |a Smania, Andrea M |e verfasserin |4 aut | |
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