Magnoflorine-Isolation and the Anticancer Potential against NCI-H1299 Lung, MDA-MB-468 Breast, T98G Glioma, and TE671 Rhabdomyosarcoma Cancer Cells
Magnoflorine (MGN) is a quaternary aporphine alkaloid that exhibits numerous therapeutic properties, including neuropsychopharmacological, anti-anxiety, immunomodulatory, anti-inflammatory, antioxidant, or antifungal activities. The aim of the present study was an investigation of the influence of MGN on viability, proliferation, induction of apoptosis, and cell cycle arrest in NCI-H1299 lung, MDA-MB-468 breast, T98G glioma, and TE671 rhabdomyosarcoma cancer cells. MGN was isolated from the roots of Berberis cretica L. by counter-current partition chromatography (CPC). Cell viability and proliferation assessments were performed by means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and 5-bromo-2'-deoxyuridine (BrDU) assays, respectively. The induction of apoptosis and cell cycle progression was measured using fluorescence-activated cell sorting analysis. MGN in high doses inhibits proliferation, induces apoptosis, and inhibits cell cycle in S/G2 phases in a dose-dependent manner. MGN seems to be a promising anti-cancer compound in therapy of some types of lung, breast, glioma, and rhabdomyosarcoma cancers, for which current standard therapies are limited or have severe strong side effects.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:10 |
|---|---|
| Enthalten in: |
Biomolecules - 10(2020), 11 vom: 10. Nov. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Okon, Estera [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 07.09.2021 Date Revised 07.09.2021 published: Electronic Citation Status MEDLINE |
|---|
| doi: |
10.3390/biom10111532 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM317502573 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM317502573 | ||
| 003 | DE-627 | ||
| 005 | 20231225163413.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/biom10111532 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1058.xml |
| 035 | |a (DE-627)NLM317502573 | ||
| 035 | |a (NLM)33182753 | ||
| 035 | |a (PII)E1532 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Okon, Estera |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Magnoflorine-Isolation and the Anticancer Potential against NCI-H1299 Lung, MDA-MB-468 Breast, T98G Glioma, and TE671 Rhabdomyosarcoma Cancer Cells |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 07.09.2021 | ||
| 500 | |a Date Revised 07.09.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Magnoflorine (MGN) is a quaternary aporphine alkaloid that exhibits numerous therapeutic properties, including neuropsychopharmacological, anti-anxiety, immunomodulatory, anti-inflammatory, antioxidant, or antifungal activities. The aim of the present study was an investigation of the influence of MGN on viability, proliferation, induction of apoptosis, and cell cycle arrest in NCI-H1299 lung, MDA-MB-468 breast, T98G glioma, and TE671 rhabdomyosarcoma cancer cells. MGN was isolated from the roots of Berberis cretica L. by counter-current partition chromatography (CPC). Cell viability and proliferation assessments were performed by means of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and 5-bromo-2'-deoxyuridine (BrDU) assays, respectively. The induction of apoptosis and cell cycle progression was measured using fluorescence-activated cell sorting analysis. MGN in high doses inhibits proliferation, induces apoptosis, and inhibits cell cycle in S/G2 phases in a dose-dependent manner. MGN seems to be a promising anti-cancer compound in therapy of some types of lung, breast, glioma, and rhabdomyosarcoma cancers, for which current standard therapies are limited or have severe strong side effects | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Berberidaceae | |
| 650 | 4 | |a Berberis cretica | |
| 650 | 4 | |a CPC | |
| 650 | 4 | |a HPLC-MS | |
| 650 | 4 | |a anti-cancer activity | |
| 650 | 4 | |a counter-current chromatography | |
| 650 | 4 | |a isoquinoline alkaloids | |
| 650 | 4 | |a magnoflorine | |
| 650 | 4 | |a natural products | |
| 650 | 7 | |a Antineoplastic Agents |2 NLM | |
| 650 | 7 | |a Aporphines |2 NLM | |
| 650 | 7 | |a Plant Extracts |2 NLM | |
| 650 | 7 | |a magnoflorine |2 NLM | |
| 650 | 7 | |a NI8K6962K4 |2 NLM | |
| 700 | 1 | |a Kukula-Koch, Wirginia |e verfasserin |4 aut | |
| 700 | 1 | |a Halasa, Marta |e verfasserin |4 aut | |
| 700 | 1 | |a Jarzab, Agata |e verfasserin |4 aut | |
| 700 | 1 | |a Baran, Marzena |e verfasserin |4 aut | |
| 700 | 1 | |a Dmoszynska-Graniczka, Magdalena |e verfasserin |4 aut | |
| 700 | 1 | |a Angelis, Apostolis |e verfasserin |4 aut | |
| 700 | 1 | |a Kalpoutzakis, Eleftherios |e verfasserin |4 aut | |
| 700 | 1 | |a Guz, Malgorzata |e verfasserin |4 aut | |
| 700 | 1 | |a Stepulak, Andrzej |e verfasserin |4 aut | |
| 700 | 1 | |a Wawruszak, Anna |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biomolecules |d 2011 |g 10(2020), 11 vom: 10. Nov. |w (DE-627)NLM228347106 |x 2218-273X |7 nnns |
| 773 | 1 | 8 | |g volume:10 |g year:2020 |g number:11 |g day:10 |g month:11 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/biom10111532 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 10 |j 2020 |e 11 |b 10 |c 11 | ||