Analytical sensitivity and clinical sensitivity of the three rapid antigen detection kits for detection of SARS-CoV-2 virus
Copyright © 2020 Elsevier B.V. All rights reserved..
BACKGROUND: Numerous rapid antigen detection (RAD) kits for diagnosing COVID-19 patients are available in the market recently.
OBJECTIVE: To compare analytical sensitivity and clinical sensitivity for the three commercially available RAD kits.
STUDY DESIGN: Analytical sensitivity for the detection of SARS-CoV-2 virus was determined by limit of detection (LOD) using RT-PCR as a reference method. Clinical sensitivity was evaluated by using respiratory specimens collected from confirmed COVID-19 patients.
RESULTS: The LOD results showed that the three RAD kits varied from 102-105 fold less sensitive than RT-PCR. Clinical sensitivity of RAD kits ranged from 22.9 %-71.4 % for detecting specimens from COVID-19 patients.
CONCLUSIONS: Although RAD kits were less sensitive than RT-PCR, understanding the clinical characteristics of different RAD kits can guide us to obtain suitable specimens for testing. The likelihood of positive results for RAD kits will be higher.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:133 |
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| Enthalten in: |
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 133(2020) vom: 16. Dez., Seite 104684 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Mak, Gannon Ck [VerfasserIn] |
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| Links: |
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| Themen: |
2019 novel coronavirus |
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| Anmerkungen: |
Date Completed 29.12.2020 Date Revised 10.01.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.jcv.2020.104684 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317438352 |
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| 100 | 1 | |a Mak, Gannon Ck |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Analytical sensitivity and clinical sensitivity of the three rapid antigen detection kits for detection of SARS-CoV-2 virus |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 29.12.2020 | ||
| 500 | |a Date Revised 10.01.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier B.V. All rights reserved. | ||
| 520 | |a BACKGROUND: Numerous rapid antigen detection (RAD) kits for diagnosing COVID-19 patients are available in the market recently | ||
| 520 | |a OBJECTIVE: To compare analytical sensitivity and clinical sensitivity for the three commercially available RAD kits | ||
| 520 | |a STUDY DESIGN: Analytical sensitivity for the detection of SARS-CoV-2 virus was determined by limit of detection (LOD) using RT-PCR as a reference method. Clinical sensitivity was evaluated by using respiratory specimens collected from confirmed COVID-19 patients | ||
| 520 | |a RESULTS: The LOD results showed that the three RAD kits varied from 102-105 fold less sensitive than RT-PCR. Clinical sensitivity of RAD kits ranged from 22.9 %-71.4 % for detecting specimens from COVID-19 patients | ||
| 520 | |a CONCLUSIONS: Although RAD kits were less sensitive than RT-PCR, understanding the clinical characteristics of different RAD kits can guide us to obtain suitable specimens for testing. The likelihood of positive results for RAD kits will be higher | ||
| 650 | 4 | |a Comparative Study | |
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 2019 novel coronavirus | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a RT-PCR | |
| 650 | 4 | |a Rapid antigen detection | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 7 | |a Antigens, Viral |2 NLM | |
| 650 | 7 | |a Reagent Kits, Diagnostic |2 NLM | |
| 700 | 1 | |a Lau, Stephen Sy |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Kitty Ky |e verfasserin |4 aut | |
| 700 | 1 | |a Chow, Nancy Ls |e verfasserin |4 aut | |
| 700 | 1 | |a Lau, C S |e verfasserin |4 aut | |
| 700 | 1 | |a Lam, Edman Tk |e verfasserin |4 aut | |
| 700 | 1 | |a Chan, Rickjason Cw |e verfasserin |4 aut | |
| 700 | 1 | |a Tsang, Dominic Nc |e verfasserin |4 aut | |
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