Downregulation of miR-10a inhibits cutaneous squamous cell carcinoma cell proliferation, migration, and invasion by targeting Syndecan-1
IJCEP Copyright © 2020..
BACKGROUND: Cutaneous squamous cell carcinoma (cSCC) is a malignancy of epidermal keratinocytes which accounts for approximately one-third of skin cancer-related death yearly. In this study, we aim to investigate the mechanism of miR-10a in regulating cellular function of cSCC cells and its possible role in prognosis of cSCC.
METHODS: The expression of miR-10a was detected by qRT-PCR. Target mRNA candidates were detected by bioinformatic analysis. Proliferative and migration capability of cSCC cell were examined by MTT assay, wound healing assay, and invasion assay, respectively. miR-10a expression was monitored in cSCC patients to elucidate the relationship between miR-10a expression and outcomes of cSCC.
RESULTS: In our study, we found that expression of miR-10a was significantly down-regulated in cSCC cell in vitro and in vivo. Moreover, our results revealed that SDC-1 was a likely target of miR-10a in regulating biologic function of cSCC cell. Additionally, miR-10a expression level was inversely correlated with cSCC cell differentiation and tumor progression.
CONCLUSION: These findings in this study indicate the importance of miR-10a in cSCC cell hallmarks and its use as a novel target for cSCC treatment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
International journal of clinical and experimental pathology - 13(2020), 10 vom: 20., Seite 2502-2512 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Xiong, Zhuyou [VerfasserIn] |
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| Themen: |
Cutaneous squamous cell carcinoma |
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| Anmerkungen: |
Date Revised 12.11.2020 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317331892 |
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| 100 | 1 | |a Xiong, Zhuyou |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Downregulation of miR-10a inhibits cutaneous squamous cell carcinoma cell proliferation, migration, and invasion by targeting Syndecan-1 |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Revised 12.11.2020 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a IJCEP Copyright © 2020. | ||
| 520 | |a BACKGROUND: Cutaneous squamous cell carcinoma (cSCC) is a malignancy of epidermal keratinocytes which accounts for approximately one-third of skin cancer-related death yearly. In this study, we aim to investigate the mechanism of miR-10a in regulating cellular function of cSCC cells and its possible role in prognosis of cSCC | ||
| 520 | |a METHODS: The expression of miR-10a was detected by qRT-PCR. Target mRNA candidates were detected by bioinformatic analysis. Proliferative and migration capability of cSCC cell were examined by MTT assay, wound healing assay, and invasion assay, respectively. miR-10a expression was monitored in cSCC patients to elucidate the relationship between miR-10a expression and outcomes of cSCC | ||
| 520 | |a RESULTS: In our study, we found that expression of miR-10a was significantly down-regulated in cSCC cell in vitro and in vivo. Moreover, our results revealed that SDC-1 was a likely target of miR-10a in regulating biologic function of cSCC cell. Additionally, miR-10a expression level was inversely correlated with cSCC cell differentiation and tumor progression | ||
| 520 | |a CONCLUSION: These findings in this study indicate the importance of miR-10a in cSCC cell hallmarks and its use as a novel target for cSCC treatment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cutaneous squamous cell carcinoma | |
| 650 | 4 | |a miR-10a | |
| 650 | 4 | |a migration | |
| 650 | 4 | |a proliferation | |
| 700 | 1 | |a Jiang, Banghong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Guangzao |e verfasserin |4 aut | |
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