SARS-CoV-2 spread across the Colombian-Venezuelan border
Copyright © 2020. Published by Elsevier B.V..
INTRODUCTION: Venezuela and Colombia both adopted measures of containment early in response to the COVID-19 pandemic. However, Venezuela's ongoing humanitarian crisis has decimated its health care system, and forced millions of Venezuelans to flee through its porous border with Colombia. The extensive shared border, and illegal cross-border transit through improvised trails between the two countries are major challenges for public health authorities. We report the first SARS-CoV-2 genomes from Venezuela, and present a snapshot of the SARS-CoV-2 epidemiologic landscape in the Colombian-Venezuelan border region.
METHODS: We sequenced and assembled viral genomes from total RNA extracted from nasopharyngeal (NP) clinical specimens using a custom reference-based analysis pipeline. Three assemblies obtained were subjected to typing using the Phylogenetic Assignment of Named Global Outbreak LINeages 'Pangolin' tool. A total of 376 publicly available SARS-CoV-2 genomes from South America were obtained from the GISAID database to perform comparative genomic analyses. Additionally, the Wuhan-1 strain was used as reference.
RESULTS: We found that two of the SARS-CoV-2 genomes from Venezuela belonged to the B1 lineage, and the third to the B.1.13 lineage. We observed a point mutation in the Spike protein gene (D614G substitution), previously reported to be associated with increased infectivity, in all three Venezuelan genomes. Additionally, three mutations (R203K/G204R substitution) were present in the nucleocapsid (N) gene of one Venezuelan genome.
CONCLUSIONS: Genomic sequencing demonstrates similarity between SARS-CoV-2 lineages from Venezuela and viruses collected from patients in bordering areas in Colombia and from Brazil, consistent with cross-border transit despite administrative measures including lockdowns. The presence of mutations associated with increased infectivity in the 3 Venezuelan genomes we report and Colombian SARS-CoV-2 genomes from neighboring borders areas may pose additional challenges for control of SARS-CoV-2 spread in the complex epidemiological landscape in Latin American countries. Public health authorities should carefully follow the progress of the pandemic and its impact on displaced populations within the region.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:86 |
|---|---|
| Enthalten in: |
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 86(2020) vom: 25. Dez., Seite 104616 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Paniz-Mondolfi, Alberto [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Border |
|---|
| Anmerkungen: |
Date Completed 21.12.2020 Date Revised 10.01.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.meegid.2020.104616 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM317253883 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM317253883 | ||
| 003 | DE-627 | ||
| 005 | 20231225162844.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.meegid.2020.104616 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1057.xml |
| 035 | |a (DE-627)NLM317253883 | ||
| 035 | |a (NLM)33157300 | ||
| 035 | |a (PII)S1567-1348(20)30447-0 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Paniz-Mondolfi, Alberto |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a SARS-CoV-2 spread across the Colombian-Venezuelan border |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 21.12.2020 | ||
| 500 | |a Date Revised 10.01.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020. Published by Elsevier B.V. | ||
| 520 | |a INTRODUCTION: Venezuela and Colombia both adopted measures of containment early in response to the COVID-19 pandemic. However, Venezuela's ongoing humanitarian crisis has decimated its health care system, and forced millions of Venezuelans to flee through its porous border with Colombia. The extensive shared border, and illegal cross-border transit through improvised trails between the two countries are major challenges for public health authorities. We report the first SARS-CoV-2 genomes from Venezuela, and present a snapshot of the SARS-CoV-2 epidemiologic landscape in the Colombian-Venezuelan border region | ||
| 520 | |a METHODS: We sequenced and assembled viral genomes from total RNA extracted from nasopharyngeal (NP) clinical specimens using a custom reference-based analysis pipeline. Three assemblies obtained were subjected to typing using the Phylogenetic Assignment of Named Global Outbreak LINeages 'Pangolin' tool. A total of 376 publicly available SARS-CoV-2 genomes from South America were obtained from the GISAID database to perform comparative genomic analyses. Additionally, the Wuhan-1 strain was used as reference | ||
| 520 | |a RESULTS: We found that two of the SARS-CoV-2 genomes from Venezuela belonged to the B1 lineage, and the third to the B.1.13 lineage. We observed a point mutation in the Spike protein gene (D614G substitution), previously reported to be associated with increased infectivity, in all three Venezuelan genomes. Additionally, three mutations (R203K/G204R substitution) were present in the nucleocapsid (N) gene of one Venezuelan genome | ||
| 520 | |a CONCLUSIONS: Genomic sequencing demonstrates similarity between SARS-CoV-2 lineages from Venezuela and viruses collected from patients in bordering areas in Colombia and from Brazil, consistent with cross-border transit despite administrative measures including lockdowns. The presence of mutations associated with increased infectivity in the 3 Venezuelan genomes we report and Colombian SARS-CoV-2 genomes from neighboring borders areas may pose additional challenges for control of SARS-CoV-2 spread in the complex epidemiological landscape in Latin American countries. Public health authorities should carefully follow the progress of the pandemic and its impact on displaced populations within the region | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Border | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a Colombia | |
| 650 | 4 | |a Coronavirus | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a Venezuela | |
| 700 | 1 | |a Muñoz, Marina |e verfasserin |4 aut | |
| 700 | 1 | |a Florez, Carolina |e verfasserin |4 aut | |
| 700 | 1 | |a Gomez, Sergio |e verfasserin |4 aut | |
| 700 | 1 | |a Rico, Angelica |e verfasserin |4 aut | |
| 700 | 1 | |a Pardo, Lisseth |e verfasserin |4 aut | |
| 700 | 1 | |a Barros, Esther C |e verfasserin |4 aut | |
| 700 | 1 | |a Hernández, Carolina |e verfasserin |4 aut | |
| 700 | 1 | |a Delgado, Lourdes |e verfasserin |4 aut | |
| 700 | 1 | |a Jaimes, Jesús E |e verfasserin |4 aut | |
| 700 | 1 | |a Pérez, Luis |e verfasserin |4 aut | |
| 700 | 1 | |a Teherán, Aníbal A |e verfasserin |4 aut | |
| 700 | 1 | |a Alshammary, Hala Alejel |e verfasserin |4 aut | |
| 700 | 1 | |a Obla, Ajay |e verfasserin |4 aut | |
| 700 | 1 | |a Khan, Zenab |e verfasserin |4 aut | |
| 700 | 1 | |a Dutta, Jayeeta |e verfasserin |4 aut | |
| 700 | 1 | |a van de Guchte, Adriana |e verfasserin |4 aut | |
| 700 | 1 | |a Gonzalez-Reiche, Ana S |e verfasserin |4 aut | |
| 700 | 1 | |a Hernandez, Matthew M |e verfasserin |4 aut | |
| 700 | 1 | |a Sordillo, Emilia Mia |e verfasserin |4 aut | |
| 700 | 1 | |a Simon, Viviana |e verfasserin |4 aut | |
| 700 | 1 | |a van Bakel, Harm |e verfasserin |4 aut | |
| 700 | 1 | |a Llewellyn, Martin S |e verfasserin |4 aut | |
| 700 | 1 | |a Ramírez, Juan David |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases |d 2001 |g 86(2020) vom: 25. Dez., Seite 104616 |w (DE-627)NLM125623070 |x 1567-7257 |7 nnns |
| 773 | 1 | 8 | |g volume:86 |g year:2020 |g day:25 |g month:12 |g pages:104616 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.meegid.2020.104616 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 86 |j 2020 |b 25 |c 12 |h 104616 | ||