Annona senegalensis extract demonstrates anticancer properties in N-diethylnitrosamine-induced hepatocellular carcinoma in male Wistar rats
Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved..
BACKGROUND: Hepatocellular carcinoma (HCC) is a common and leading cancer around the globe. This study investigated the anticancer properties of extract of Annona senegalensis in N-diethylnitrosamine (DEN) - induced hepatocellular carcinoma in male Wistar rats.
METHODS: Rats were simultaneously induced with a combination of 100 mg/kg b.wt of DEN and 0.5 mL/kg of carbon tetrachloride (CCl4) intraperitoneally once a week for three weeks in a row. Thereafter, animals were treated with 100 mg/kg and 200 mg/kg b.wt of A. senegalensis extract daily for 21days. Analysis using gas chromatography-mass spectrometry (GC-MS) was carried out to discover the phytoconstituents contained in the n-hexane extract of A. senegelensis. The levels of liver function parameters and antioxidant enzyme activities were determined via spectrophotometric analysis. Reverse transcriptase-polymerase chain reaction technique was used to assess the gene expression patterns of BCL-2, P53, P21, IL-6, FNTA, VEGF, HIF, AFP, XIAP, and EGFR mRNAs.
RESULTS: Treatment of DEN-induced hepatocellular carcinoma Wistar rats with the extract caused significant (p < 0.05) decrease in the activities of ALT and AST. It also resulted in a reduction of the concentration of MDA and a significant increase (p < 0.05) in SOD and GSH activities. IL-6, BCL-2, VEGF, EGFR, XIAP, FNTA, and P21 mRNAs expressions were significantly (p < 0.05) downregulated after treatment. Histopathological analysis revealed that the extract improved the liver architecture.
CONCLUSION: A. senegelensis n-hexane extract demonstrates its anticancer properties by improving the liver architecture, increasing the antioxidant defense systems, downregulating the pro-inflammatory, anti-apoptotic, angiogenic, alpha-fetoprotein and farnesyl transferase mRNAs expression and hitherto up-regulate the expression of tumor suppressor (P21 and P53) mRNAs.
Medienart: |
E-Artikel |
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Erscheinungsjahr: |
2020 |
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Erschienen: |
2020 |
Enthalten in: |
Zur Gesamtaufnahme - volume:131 |
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Enthalten in: |
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie - 131(2020) vom: 01. Nov., Seite 110786 |
Sprache: |
Englisch |
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Beteiligte Personen: |
Yakubu, Omolara F [VerfasserIn] |
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Links: |
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Anmerkungen: |
Date Completed 19.02.2021 Date Revised 19.02.2021 published: Print-Electronic Citation Status MEDLINE |
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doi: |
10.1016/j.biopha.2020.110786 |
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funding: |
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Förderinstitution / Projekttitel: |
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PPN (Katalog-ID): |
NLM317210785 |
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245 | 1 | 0 | |a Annona senegalensis extract demonstrates anticancer properties in N-diethylnitrosamine-induced hepatocellular carcinoma in male Wistar rats |
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500 | |a published: Print-Electronic | ||
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520 | |a Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved. | ||
520 | |a BACKGROUND: Hepatocellular carcinoma (HCC) is a common and leading cancer around the globe. This study investigated the anticancer properties of extract of Annona senegalensis in N-diethylnitrosamine (DEN) - induced hepatocellular carcinoma in male Wistar rats | ||
520 | |a METHODS: Rats were simultaneously induced with a combination of 100 mg/kg b.wt of DEN and 0.5 mL/kg of carbon tetrachloride (CCl4) intraperitoneally once a week for three weeks in a row. Thereafter, animals were treated with 100 mg/kg and 200 mg/kg b.wt of A. senegalensis extract daily for 21days. Analysis using gas chromatography-mass spectrometry (GC-MS) was carried out to discover the phytoconstituents contained in the n-hexane extract of A. senegelensis. The levels of liver function parameters and antioxidant enzyme activities were determined via spectrophotometric analysis. Reverse transcriptase-polymerase chain reaction technique was used to assess the gene expression patterns of BCL-2, P53, P21, IL-6, FNTA, VEGF, HIF, AFP, XIAP, and EGFR mRNAs | ||
520 | |a RESULTS: Treatment of DEN-induced hepatocellular carcinoma Wistar rats with the extract caused significant (p < 0.05) decrease in the activities of ALT and AST. It also resulted in a reduction of the concentration of MDA and a significant increase (p < 0.05) in SOD and GSH activities. IL-6, BCL-2, VEGF, EGFR, XIAP, FNTA, and P21 mRNAs expressions were significantly (p < 0.05) downregulated after treatment. Histopathological analysis revealed that the extract improved the liver architecture | ||
520 | |a CONCLUSION: A. senegelensis n-hexane extract demonstrates its anticancer properties by improving the liver architecture, increasing the antioxidant defense systems, downregulating the pro-inflammatory, anti-apoptotic, angiogenic, alpha-fetoprotein and farnesyl transferase mRNAs expression and hitherto up-regulate the expression of tumor suppressor (P21 and P53) mRNAs | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a Annona senegalensis | |
650 | 4 | |a Gene expression | |
650 | 4 | |a Hepatocellular carcinoma | |
650 | 4 | |a N-Diethylnitrosamine | |
650 | 7 | |a Antineoplastic Agents, Phytogenic |2 NLM | |
650 | 7 | |a Antioxidants |2 NLM | |
650 | 7 | |a Plant Extracts |2 NLM | |
650 | 7 | |a Diethylnitrosamine |2 NLM | |
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650 | 7 | |a Carbon Tetrachloride |2 NLM | |
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700 | 1 | |a Metibemu, Damilohun S |e verfasserin |4 aut | |
700 | 1 | |a Adelani, Isaacson B |e verfasserin |4 aut | |
700 | 1 | |a Adesina, Gbolahan O |e verfasserin |4 aut | |
700 | 1 | |a Edokwe, Chidera B |e verfasserin |4 aut | |
700 | 1 | |a Oseha, Oyinkansola E |e verfasserin |4 aut | |
700 | 1 | |a Adebayo, Abiodun H |e verfasserin |4 aut | |
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