SMN Protein Contributes to Skeletal Muscle Cell Maturation Via Caspase-3 and Akt Activation
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved..
BACKGROUND/AIM: In spinal muscular atrophy (SMA), systemic deficiency of survival motor neurons (SMN) caused by loss or mutation of SMN1 leads to SMA symptoms. SMA was, for a long time, considered as a selective motor-neuron disease. However, accumulated evidence suggests that skeletal muscle cells are affected by low levels of SMN protein. The purpose of this study was to elucidate the function of SMN protein in skeletal cell differentiation and maturation.
MATERIALS AND METHODS: In SMNΔ7 mice, which exhibit a systemic reduction of SMN protein, muscle atrophy was evaluated. To direct the effect of SMN against muscle cells, SMN functions were examined by knockdown of SMN in mouse myoblasts cell line C2C12 using siRNA.
RESULTS: SMNΔ7 mice showed muscle atrophy accompanied by decreased both expression of a myogenesis marker and a proliferating marker. In SMN-knockdown myoblasts, early expression of myosin heavy chain and reduced multinuclear myotube formation were found. Decreased caspase-3 activity and reduced phosphorylation of Akt were observed at an early stage of differentiation in SMN-knockdown myoblasts.
CONCLUSION: A critical role of SMN protein in muscle cell differentiation via caspase-3 and Akt activation was shown.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2020 |
|---|---|
| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:34 |
|---|---|
| Enthalten in: |
In vivo (Athens, Greece) - 34(2020), 6 vom: 03. Nov., Seite 3247-3254 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Ando, Shiori [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 18.06.2021 Date Revised 31.05.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.21873/invivo.12161 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM317126881 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM317126881 | ||
| 003 | DE-627 | ||
| 005 | 20231225162605.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2020 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.21873/invivo.12161 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1057.xml |
| 035 | |a (DE-627)NLM317126881 | ||
| 035 | |a (NLM)33144430 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Ando, Shiori |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a SMN Protein Contributes to Skeletal Muscle Cell Maturation Via Caspase-3 and Akt Activation |
| 264 | 1 | |c 2020 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 18.06.2021 | ||
| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. | ||
| 520 | |a BACKGROUND/AIM: In spinal muscular atrophy (SMA), systemic deficiency of survival motor neurons (SMN) caused by loss or mutation of SMN1 leads to SMA symptoms. SMA was, for a long time, considered as a selective motor-neuron disease. However, accumulated evidence suggests that skeletal muscle cells are affected by low levels of SMN protein. The purpose of this study was to elucidate the function of SMN protein in skeletal cell differentiation and maturation | ||
| 520 | |a MATERIALS AND METHODS: In SMNΔ7 mice, which exhibit a systemic reduction of SMN protein, muscle atrophy was evaluated. To direct the effect of SMN against muscle cells, SMN functions were examined by knockdown of SMN in mouse myoblasts cell line C2C12 using siRNA | ||
| 520 | |a RESULTS: SMNΔ7 mice showed muscle atrophy accompanied by decreased both expression of a myogenesis marker and a proliferating marker. In SMN-knockdown myoblasts, early expression of myosin heavy chain and reduced multinuclear myotube formation were found. Decreased caspase-3 activity and reduced phosphorylation of Akt were observed at an early stage of differentiation in SMN-knockdown myoblasts | ||
| 520 | |a CONCLUSION: A critical role of SMN protein in muscle cell differentiation via caspase-3 and Akt activation was shown | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Myotube formation | |
| 650 | 4 | |a skeletal muscles | |
| 650 | 4 | |a spinal muscular atrophy | |
| 650 | 4 | |a survival motor neurons | |
| 650 | 7 | |a Smn1 protein, mouse |2 NLM | |
| 650 | 7 | |a Survival of Motor Neuron 1 Protein |2 NLM | |
| 650 | 7 | |a Proto-Oncogene Proteins c-akt |2 NLM | |
| 650 | 7 | |a EC 2.7.11.1 |2 NLM | |
| 650 | 7 | |a Caspase 3 |2 NLM | |
| 650 | 7 | |a EC 3.4.22.- |2 NLM | |
| 700 | 1 | |a Tanaka, Miruto |e verfasserin |4 aut | |
| 700 | 1 | |a Chinen, Naoki |e verfasserin |4 aut | |
| 700 | 1 | |a Nakamura, Shinsuke |e verfasserin |4 aut | |
| 700 | 1 | |a Shimazawa, Masamitsu |e verfasserin |4 aut | |
| 700 | 1 | |a Hara, Hideaki |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t In vivo (Athens, Greece) |d 1992 |g 34(2020), 6 vom: 03. Nov., Seite 3247-3254 |w (DE-627)NLM012776912 |x 1791-7549 |7 nnns |
| 773 | 1 | 8 | |g volume:34 |g year:2020 |g number:6 |g day:03 |g month:11 |g pages:3247-3254 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.21873/invivo.12161 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 34 |j 2020 |e 6 |b 03 |c 11 |h 3247-3254 | ||