Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis
Copyright © 2020 Elsevier Ltd. All rights reserved..
The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose. The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:251 |
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| Enthalten in: |
Carbohydrate polymers - 251(2021) vom: 01. Jan., Seite 117078 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Zhang, Hua [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 23.04.2021 Date Revised 23.04.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.carbpol.2020.117078 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM317108840 |
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| 245 | 1 | 0 | |a Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2020 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose. The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a Pinecones | |
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| 650 | 4 | |a Polysaccharide | |
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| 650 | 7 | |a Galactose |2 NLM | |
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| 700 | 1 | |a Regenstein, Joe Mac |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xin |e verfasserin |4 aut | |
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