Study on visfatin-induced inflammation and necroptosis via LOX-1 in human umbilical vein endothelial cells
The aim of the study is to identify the effects and underlying mechanisms of visfatin on inflammation and necroptosis in vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with visfatin or pretreated with Polyinosinic acid (LOX-1 inhibitor). By using the Western blot, RT-PCR, immunocytochemistry, enzyme-linked immunosorbent assay (ELISA), MTT and flow cytometry technique, the occurrence of inflammation and necroptosis in HUVECs were evaluated. Our results showed that 100 ng/mL visfatin significantly increased the mRNA and protein expression of monocyte chemotactic protein 1 (MCP-1) and LOX-1 after 24 hours' treatment in HUVECs. However, pretreatment with Polyinosinic acid could significantly reduce the expression of MCP-1 compared with visfatin group. Additionally, 100 ng/mL visfatin could induce the production of necrotic features and increase the mRNA expression of BMF (one of the markers of necroptosis), while pretreating with Polyinosinic acid markedly downregulated the mRNA expression of BMF gene and promoted the cell proliferation. These results indicate that visfatin might induce inflammation and necroptosis via LOX-1 in HUVECs, suggesting that visfatin plays a central role in the development of atherosclerosis.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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| Enthalten in: |
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi - 37(2020), 5 vom: 25. Okt., Seite 834-841 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Han, Xiaoyu [VerfasserIn] |
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| Links: |
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| Themen: |
EC 2.4.2.12 |
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| Anmerkungen: |
Date Completed 15.01.2021 Date Revised 09.08.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.7507/1001-5515.202003067 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM317089129 |
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| 245 | 1 | 0 | |a Study on visfatin-induced inflammation and necroptosis via LOX-1 in human umbilical vein endothelial cells |
| 264 | 1 | |c 2020 | |
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| 500 | |a Date Completed 15.01.2021 | ||
| 500 | |a Date Revised 09.08.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The aim of the study is to identify the effects and underlying mechanisms of visfatin on inflammation and necroptosis in vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were stimulated with visfatin or pretreated with Polyinosinic acid (LOX-1 inhibitor). By using the Western blot, RT-PCR, immunocytochemistry, enzyme-linked immunosorbent assay (ELISA), MTT and flow cytometry technique, the occurrence of inflammation and necroptosis in HUVECs were evaluated. Our results showed that 100 ng/mL visfatin significantly increased the mRNA and protein expression of monocyte chemotactic protein 1 (MCP-1) and LOX-1 after 24 hours' treatment in HUVECs. However, pretreatment with Polyinosinic acid could significantly reduce the expression of MCP-1 compared with visfatin group. Additionally, 100 ng/mL visfatin could induce the production of necrotic features and increase the mRNA expression of BMF (one of the markers of necroptosis), while pretreating with Polyinosinic acid markedly downregulated the mRNA expression of BMF gene and promoted the cell proliferation. These results indicate that visfatin might induce inflammation and necroptosis via LOX-1 in HUVECs, suggesting that visfatin plays a central role in the development of atherosclerosis | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a LOX-1 | |
| 650 | 4 | |a human umbilical vein endothelial cells | |
| 650 | 4 | |a inflammation | |
| 650 | 4 | |a necroptosis | |
| 650 | 4 | |a visfatin | |
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| 650 | 7 | |a Scavenger Receptors, Class E |2 NLM | |
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| 700 | 1 | |a Liu, Xiaojing |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Ye |e verfasserin |4 aut | |
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| 773 | 1 | 8 | |g volume:37 |g year:2020 |g number:5 |g day:25 |g month:10 |g pages:834-841 |
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