An Animal Able To Tolerate D2 O
© 2020 Wiley-VCH GmbH..
It is possible to gain a deeper insight into the role of water in biology by using physicochemical variant molecules, such as deuterium oxide (D2 O); however, D2 O is toxic to multicellular organisms in high concentrations. By using a unique desiccation-rehydration process, we demonstrate that the anhydrobiotic nematode Panagrolaimus superbus is able to tolerate and proliferate in 99 % D2 O. Moreover, we analysed P. superbus' water-channel protein (aquaporin; AQP), which is associated with dehydration/rehydration, by comparing its primary structure and modelling its tertiary structure in silico. Our data evidence that P. superbus' AQP is an aquaglyceroporin, a class of water channel known to display a wider pore; this helps to explain the rapid and successful organismal influx of D2 O into this species. This is the first demonstration of an animal able to withstand high D2 O levels, thus paving a way for the investigation of the effects D2 O on higher levels of biological organization.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:22 |
|---|---|
| Enthalten in: |
Chembiochem : a European journal of chemical biology - 22(2021), 6 vom: 16. März, Seite 988-991 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
de Carli, Gabriel José [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 19.11.2021 Date Revised 19.11.2021 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1002/cbic.202000642 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM316943037 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM316943037 | ||
| 003 | DE-627 | ||
| 005 | 20231225162158.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1002/cbic.202000642 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1056.xml |
| 035 | |a (DE-627)NLM316943037 | ||
| 035 | |a (NLM)33125805 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a de Carli, Gabriel José |e verfasserin |4 aut | |
| 245 | 1 | 3 | |a An Animal Able To Tolerate D2 O |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 19.11.2021 | ||
| 500 | |a Date Revised 19.11.2021 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2020 Wiley-VCH GmbH. | ||
| 520 | |a It is possible to gain a deeper insight into the role of water in biology by using physicochemical variant molecules, such as deuterium oxide (D2 O); however, D2 O is toxic to multicellular organisms in high concentrations. By using a unique desiccation-rehydration process, we demonstrate that the anhydrobiotic nematode Panagrolaimus superbus is able to tolerate and proliferate in 99 % D2 O. Moreover, we analysed P. superbus' water-channel protein (aquaporin; AQP), which is associated with dehydration/rehydration, by comparing its primary structure and modelling its tertiary structure in silico. Our data evidence that P. superbus' AQP is an aquaglyceroporin, a class of water channel known to display a wider pore; this helps to explain the rapid and successful organismal influx of D2 O into this species. This is the first demonstration of an animal able to withstand high D2 O levels, thus paving a way for the investigation of the effects D2 O on higher levels of biological organization | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a anhydrobiosis | |
| 650 | 4 | |a aquaporin | |
| 650 | 4 | |a desiccation tolerance | |
| 650 | 4 | |a deuterium oxide | |
| 650 | 7 | |a Aquaporins |2 NLM | |
| 650 | 7 | |a Helminth Proteins |2 NLM | |
| 650 | 7 | |a Deuterium Oxide |2 NLM | |
| 650 | 7 | |a J65BV539M3 |2 NLM | |
| 700 | 1 | |a Contiliani, Danyel Fernandes |e verfasserin |4 aut | |
| 700 | 1 | |a Giuliatti, Silvana |e verfasserin |4 aut | |
| 700 | 1 | |a Pereira, Tiago Campos |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Chembiochem : a European journal of chemical biology |d 2000 |g 22(2021), 6 vom: 16. März, Seite 988-991 |w (DE-627)NLM117153788 |x 1439-7633 |7 nnns |
| 773 | 1 | 8 | |g volume:22 |g year:2021 |g number:6 |g day:16 |g month:03 |g pages:988-991 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1002/cbic.202000642 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 22 |j 2021 |e 6 |b 16 |c 03 |h 988-991 | ||