Identification of the hydantoin alkaloids parazoanthines as novel CXCR4 antagonists by computational and in vitro functional characterization
Copyright © 2020 Elsevier Inc. All rights reserved..
CXCR4 chemokine receptor represents an attractive pharmacological target due to its key role in cancer metastasis and inflammatory diseases. Starting from our previously-developed pharmacophoric model, we applied a combined computational and experimental approach that led to the identification of the hydantoin alkaloids parazoanthines, isolated from the Mediterranean Sea anemone Parazoanthus axinellae, as novel CXCR4 antagonists. Parazoanthine analogues were then synthesized to evaluate the contribution of functional groups to the overall activity. Within the panel of synthesized natural and non-natural parazoanthines, parazoanthine-B was identified as the most potent CXCR4 antagonist with an IC50 value of 9.3 nM, even though all the investigated compounds were able to antagonize in vitro the down-stream effects of CXC12, albeit with variable potency and efficacy. The results of our study strongly support this class of small molecules as potent CXCR4 antagonists in tumoral pathologies characterized by an overexpression of this receptor. Furthermore, their structure-activity relationships allowed the optimization of our pharmacophoric model, useful for large-scale in silico screening.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2020 |
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| Erschienen: |
2020 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:105 |
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| Enthalten in: |
Bioorganic chemistry - 105(2020) vom: 21. Dez., Seite 104337 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Vitale, Rosa Maria [VerfasserIn] |
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| Links: |
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| Themen: |
Alkaloids |
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| Anmerkungen: |
Date Completed 19.03.2021 Date Revised 19.03.2021 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.bioorg.2020.104337 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM316820172 |
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| 520 | |a CXCR4 chemokine receptor represents an attractive pharmacological target due to its key role in cancer metastasis and inflammatory diseases. Starting from our previously-developed pharmacophoric model, we applied a combined computational and experimental approach that led to the identification of the hydantoin alkaloids parazoanthines, isolated from the Mediterranean Sea anemone Parazoanthus axinellae, as novel CXCR4 antagonists. Parazoanthine analogues were then synthesized to evaluate the contribution of functional groups to the overall activity. Within the panel of synthesized natural and non-natural parazoanthines, parazoanthine-B was identified as the most potent CXCR4 antagonist with an IC50 value of 9.3 nM, even though all the investigated compounds were able to antagonize in vitro the down-stream effects of CXC12, albeit with variable potency and efficacy. The results of our study strongly support this class of small molecules as potent CXCR4 antagonists in tumoral pathologies characterized by an overexpression of this receptor. Furthermore, their structure-activity relationships allowed the optimization of our pharmacophoric model, useful for large-scale in silico screening | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CXCR4 antagonists | |
| 650 | 4 | |a Hydantoin alkaloids | |
| 650 | 4 | |a Molecular docking | |
| 650 | 4 | |a Natural products | |
| 650 | 4 | |a Parazoanthines | |
| 650 | 4 | |a Pharmacophoric model | |
| 650 | 7 | |a Alkaloids |2 NLM | |
| 650 | 7 | |a Hydantoins |2 NLM | |
| 650 | 7 | |a Receptors, CXCR4 |2 NLM | |
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| 700 | 1 | |a Tinto, Francesco |e verfasserin |4 aut | |
| 700 | 1 | |a Solari, Agnese |e verfasserin |4 aut | |
| 700 | 1 | |a Gatti, Monica |e verfasserin |4 aut | |
| 700 | 1 | |a Nuzzo, Genoveffa |e verfasserin |4 aut | |
| 700 | 1 | |a Ioannou, Efstathia |e verfasserin |4 aut | |
| 700 | 1 | |a Roussis, Vassilios |e verfasserin |4 aut | |
| 700 | 1 | |a Ciavatta, Maria Letizia |e verfasserin |4 aut | |
| 700 | 1 | |a Manzo, Emiliano |e verfasserin |4 aut | |
| 700 | 1 | |a Florio, Tullio |e verfasserin |4 aut | |
| 700 | 1 | |a Amodeo, Pietro |e verfasserin |4 aut | |
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